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| 血清HMGB1 WISP1对结肠癌患者术后淋巴结转移的预测价值 |
| The predictive value of serum HMGB1 and WISP1 for postoperative lymph node metastasis in colon cancer patients |
| 投稿时间：2025-07-07 |
| DOI：10.3969/j.issn.1000-0399.2026.08.015 |
| 中文关键词: 结肠癌 高迁移率族蛋白B1 Wnt1诱导型信号通路蛋白1 淋巴结转移 预测 |
| 英文关键词: Colon cancer High mobility group protein B1 WNT1-inducible-signaling pathway protein 1 Lymph node metastasis Prediction |
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| 中文摘要: |
| 目的 探讨血清高迁移率族蛋白B1（HMGB1）、Wnt1诱导型信号通路蛋白1（WISP1）对结肠癌患者术后淋巴结转移的预测价值。方法 选取2023年1月至2024年12月在南阳南石医院收治的107例结肠癌患者为结肠癌组，另纳入73例结肠良性病变患者为良性病变组，同期纳入58例健康志愿者为对照组。采用酶联免疫吸附测定法（ELISA）检测血清HMGB1、WISP1水平；多因素logistic回归分析影响淋巴结转移的因素，受试者工作特征（ROC）曲线分析血清HMGB1、WISP1水平对淋巴结转移的预测价值。另选取2024年6月至2025年1月在本院就诊的61例结肠癌患者进行外部验证。结果 结肠癌组血清HMGB1、WISP1水平均高于对照组和良性病变组，良性病变组血清HMGB1、WISP1水平均高于对照组（P<0.05）;107例结肠癌患者中，术后发生淋巴结转移29例，占所有结肠癌患者的27.10%，未发生淋巴结转移78例，占所有结肠癌患者的72.90%；相比于未转移组，转移组血清HMGB1、WISP1水平以及侵及浆膜层的占比均较高（t=8.698、7.896、7.210,P<0.05）；血清HMGB1、WISP1水平升高以及浸润深度为侵及浆膜层是影响发生淋巴结转移的危险因素（P<0.05）；血清HMGB1、WISP1二者联合预测术后淋巴结转移的曲线下面积（AUC）为0.951，二者联合优于各自单独预测(Z二者联合-HMGB1=2.603、Z二者联合-WISP1=2.571,P<0.05)；预测模型在外部验证中的AUC为0.939，灵敏度为94.74%，特异度为83.33%。结论 结肠癌患者血清HMGB1、WISP1水平升高，HMGB1、WISP1与结肠癌患者术后淋巴结转移密切相关，二者联合检测对预测术后淋巴结转移有较高临床应用效能。 |
| 英文摘要: |
| Objective To investigate the predictive value of serum high-mobility group box 1（HMGB1） and Wnt1-inducible signaling pathway protein 1（WISP1） for postoperative lymph node metastasis in patients with colon cancer. Methods A total of 107 patients with colorectal cancer admitted to Nanyang Nanshi Hospital between January 2023 and December 2024 were selected as the colorectal cancer group. In addition, 73 patients with benign colorectal lesions were recruited as the benign lesion group, and 58 healthy volunteers were enrolled as the control group during the same period. Enzyme-linked immunosorbent assay（ELISA） was used to measure serum levels of HMGB1 and WISP1. Multivariate Logistic regression was employed to analyse factors influencing lymph node metastasis. ROC curve was plotted to analyse the predictive value of serum HMGB1 and WISP1 levels for lymph node metastasis. Sixty-one patients with colorectal cancer who were treated at our hospital between June 2024 and January 2025 were selected for external validation. Results Serum HMGB1 and WISP1 levels in the colon cancer group were higher than those in the control group and the benign lesion group, and levels in the benign lesion group were higher than those in the control group（P<0.05）. Among the 107 patients with colon cancer, postoperative lymph node metastasis occurred in 29 cases（27.10%）, while 78 cases（72.90%） showed no lymph node metastasis. Compared with the non-metastasis group, the metastasis group had higher serum HMGB1 and WISP1 levels and a higher proportion of serosal invasion（t=8.698, 7.896, 7.210, P<0.05）. Elevated serum levels of HMGB1 and WISP1, as well as depth of invasion and involvement of the serosal layer, were risk factors for lymph node metastasis（P<0.05）. The AUC for the combined prediction of postoperative lymph node metastasis using serum HMGB1 and WISP1 was 0.951, the combined model outperformed the individual models(Zunion-HMGB1=2.603, Zunion-WISP1=2.571, P<0.05). The AUC of the prediction model in external validation was 0.939, with a sensitivity of 94.74% and a specificity of 83.33%. Conclusion Serum HMGB1 and WISP1 levels are elevated in patients with colon cancer and are closely associated with postoperative lymph node metastasis. Combined detection of HMGB1 and WISP1 shows high clinical efficacy in predicting postoperative lymph node metastasis. |
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