| Objective To assess the biocompatibility of three kinds of endodontic bioactive materials, i.e., mineral trioxide aggregate (MTA), bioaggrregate (BA) and biodentine (BD), and to investigate the effects of these cements on osteogenic differentiation of the Mus musculus mesenchymal stem cells (MSCs). Methods The cell viability, mineralization and differentiation ability of Mus musculus MSCs were evaluated by XTT assay and ALP staining, and the effects of MTA, BA and BD on osteogenic differentiation of the MSCs were observed respectively. Results The cell viability of MSCs in various concentrations of BD (1, 1/2 and 1/4) were all significantly lower than that of MTA and BA (P<0.001), but there was no significant difference in the cell viability among the three cements when their concentrations dropped to 1/10 and 1/50. In the ALP staining indicating mineralization and differentiation ability of MSCs, MTA, BA and low concentration of BD all led to an increase in the tinctural value when compared with the controls (P<0.05). Conclusion MTA, BA and low concentration of BD have good biocompatibility with the Mus musculus MSCs. Meanwhile, they have motive effects of mineralization and differentiation during the course of osteogenic differentiation of the murine MSCs, suggesting that these cements may be used as the root-end filling material of root canal. |
