| Objective To discuss the adaption phenomenon to hepatic injury induced by rifampicin in rats and the possible role or process oxidative stress might be involved in.Methods The laboratory rats in the control group and the rifampicin group (each n=16) were fed by lavage once daily with 0.9% normal saline 10 mL/(kg·d) or rifampicin suspension 100 mg/(kg·d), respectively.During the course of 7-week successive drug lavage, the serum levels of transaminase, bilirubin and ALP of rats were dynamically monitored at the first, 14th, 28th, 49th day.When 4 rats in each group were killed at the 14th day, and the rest rats being killed at the 49th day, their hepatic histopathological changes were observed and their hepatic levels of SOD, MDA and GSH-PX were measured, respectively.Results Compared with the control group, the serum levels of ALT, AST, TB and DB of rats in the rifampicin group reached its peak at the 14th day, followed by a trend of slow decline (P<0.05), and at the 49th day there was no significant difference between the two groups (P>0.05).The rifampicin group showed much higher level of hepatic MDA and much lower levels of hepatic SOD and GPX in comparison with the control group (P<0.05).The gross liver samples of rats in the rifampicin group were found to be stained yellow, and further under microscopy, the hepatic steatosis, mild necrosis and inflammation were observed.Conclusion Administration of rifampicin could lead to the adaption phenomenon to hepatic injury in rats, and oxidative stress might be involved in such process. |
