| Objective To establish and compare mouse models in order to evaluate drugs for nonalcoholic fatty liver disease (NAFLD). Methods Thirty-five Kunming mice were divided intofour groups,namely, normal control group, high-fat diet group, high-fat and fructose diet group, high-fat and fructose plus hydrocortisone group.After four week treatment, the changes in mouse body weight, animal activity, liver index, serum biochemical indexes and liver pathology were detected and analyzed. Results Compared with normal control group, the weight increased most obviously in the high fat group, followed by high fat-fructose group, andthe weight of the high fat-fructose plus hydrocortisonewaslower thanthat of the normal control group(P<0.05). Andtheliver index in three models was higher thanthat of the normal control group(P<0.05). The serum glucose, triglyceride level, hepatic triglyceride were significantly higher than those in the normal control group(P<0.05). Among threemodels groups the histopathologic injury in mouse liver tissuesappeared the most serious on hepatic steatosis in the highfat-fructose plus hydrocortisone group,which was optimal method of established model. Conclusion NAFLD model can be prepared by different treatment methods, but the degree of liver damage is different. |
