| Objective To explore the correlation between the changes of matrix metal loproteinases(MMP-2,MMP-9) and the coronary artery lesion (CAL)by echocardiography and pathological detection in a murine model of mice with Kawasaki disease.Methods Lactobacillus casei cell wall extract(LCWE) was extracted and diluted to 2.5 mg/ML,which was then used for immunization of genetically predisposed mice to establish animal model of coronary artery.Eighty BALB/C mice(4~6 weeks old) were randomly divided into experimental group and control group,with 40 mice in each group.The experimental group was injected with LCWE 0.4mL(1mg), while the control group with the same amount of phosphate buffer solution(PBS). The general condition and the weight of mice were observed and recorded.Orbital blood samples were collected respectively on 1st,3rd,7th and 28th day after administration, which were used to measure the concentration of MMP-2 and MMP-9.The heart and liver samples were collected for pathological section to investigate the histopathological changes.Results The BW,HW and HW/BW in experimental group were obviously lower than those in control group on 3rd and 7th day(P<0.05).The concentration of MMP-2,MMP-9 gradually increased in the earlier stage in experimental group,and the level decreased significantly on 28th day,while the concentration of MMP-2,MMP-9 showed no significant change in control group(P<0.05).Conclusions Mice can be infected with LCWE,and MMP-2,MMP-9 are fine index to reflect CAL of KD, which might be used for the early clinical diagnosis of KD patients. |
