| Objective To investigate the effect of resveratrol (Res) on apoptosis and telomerase activity of A549 cells of lung adenocarcinoma. Methods Human lung adenocarcinoma A549 cells purchased from Institute of Cell Biology, Chinese Academy of Sciences were cultured routinely to logarithmic growth phase and treated with Res at different concentrations, with the final concentration of 3, 10, 30, 60, 100, 200, 300mg/L, respectively), and the control group was Res (0mg/L). The effect of Res on lung adenocarcinoma A549 cell absorbance values (A values) was determined by MTT assay, and the cell growth inhibitory rate was calculated. The effect of Res on apoptotic rate was determined by Annexin-Ⅴ/PI double staining. Telomerase mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR), and the relative activity of telomerase and β-actin was calculated. Results Res inhibited the proliferation of A549 cells in a dose-dependent manner. Compared with control group, the proliferation of lung adenocarcinoma A549 cells was significantly inhibited by different concentrations of Res treatment (10, 30, 100 mg/L). Annexin-Ⅴ/PI results indicated that the number of apoptotic cells was significantly up-regulated with the increase of Res dose (10, 30, 100 mg/L). Compared with control group, the relative mRNA content of telomerase decreased significantly after Res (10, 30, 100 mg/L) treatment, and the difference was statistically significant (P<0.05). Conclusion Res can induce apoptosis of lung adenocarcinoma A549 cells, and its mechanism may be related to the inhibition of telomerase activity. |
