文章摘要
新生儿缺血缺氧性脑病患儿血清CCL5 GM-CSF表达及其与预后相关性
Expressions of serum CCL5 and GM-CSF in neonates with hypoxic-ischemic encephalopathy and their correlation with prognosis
投稿时间：2020-08-18  
DOI：10.3969/j.issn.1000-0399.2021.01.008
中文关键词: 新生儿缺血缺氧性脑病患儿  趋化因子5  粒细胞-巨噬细胞集落刺激因子  预后
英文关键词: Children with neonatal hypoxic ischemic encephalopathy  Chemokine 5  Granulocyte macrophage colony stimulating factor  Prognosis
基金项目:
作者单位
韦伟 570100 海南海口 海南省妇女儿童医学中心新生儿科 
轩妍 570100 海南海口 海南省妇女儿童医学中心新生儿科 
许文婷 570100 海南海口 海南省妇女儿童医学中心新生儿科 
曹祥 570100 海南海口 海南省妇女儿童医学中心新生儿科 
摘要点击次数: 1285
全文下载次数: 0
中文摘要:
      目的 探究新生儿缺血缺氧性脑病（HIE）患儿血清趋化因子5（CCL5）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）的表达水平及与预后的关系。方法 选取2015年1月至2019年5月在海南省妇女儿童医学中心诊治的82例HIE患儿作为患病组，根据患儿病情严重程度，分为轻度组（31例）、中度组（26例）与重度组（25例），另取同期60例健康新生儿作为健康组。采用酶联免疫吸附法检测受试者血清中CCL5、GM-CSF的表达水平，采用新生儿神经行为测定（NBNA）评分评估各组新生儿的神经系统的发育情况，分析血清CCL5、GM-CSF之间及二者与NBNA评分的相关性；采用格塞尔发育量表（GDS）评估患儿预后情况，并分析其与CCL5、GM-CSF表达的关系。结果 HIE患儿血清CCL5、GM-CSF的表达水平分别为（83.41±；10.95）ng/L、（52.27±；11.71）pg/mL，与健康组相比，差异有统计学意义（P<0.05）。随着病情加重，HIE患儿血清中CCL5表达水平升高，GM-CSF表达水平下降，差异有统计学意义（P<0.05）。HIE患儿血清CCL5与NBNA评分呈负相关（r=-0.318，P<0.05），GM-CSF水平与NBNA评分呈正相关（r=0.338，P<0.05），CCL5水平与GM-CSF水平呈负相关（r=-0.359，P<0.05）。对HIE患儿随访1年，CCL5高表达患儿GDS评分低于CCL5低表达患儿， GM-CSF高表达患儿GDS评分高于GM-CSF低表达患儿，差异有统计学意义（P<0.05）。结论 血清CCL5在HIE患儿中表达上调，GM-CSF在HIE患儿中表达下调，二者水平与HIE患儿预后有关，可能作为评价HIE患儿预后的潜在标志物。
英文摘要:
      Objective To investigate the expression levels of serum chemokine 5 (CCL5) and granulocyte macrophage colony stimulating factor (GM-CSF) in children with neonatal hypoxic ischemic encephalopathy (HIE) and their relationship with prognosis. Methods A total of 82 cases of HIE children diagnosed and treated in Hainan Women and Children Medical Center from January 2015 to May 2019 were selected as the disease group;according to the severity of the disease, they were divided into mild group (31 cases), moderate group (26 cases) and severe group (25 cases). Another 60 healthy newborns with normal physical examination in the same period were selected as the healthy group. Enzyme linked immunosorbent assay was used to detect the expression levels of CCL5 and GM-CSF in serum.Neonatal Behavioral Neurological Assessment (NBNA) score was used to evaluate the development of nervous system in each group.Pearson method was used to analyze the correlation between serum CCL5, GM-CSF and NBNA score.The prognosis was evaluated by Gesell developmental test scales (GDS), and the relationship between the prognosis and the expression of CCL5 and GM-CSF was analyzed. Results The expression levels of serum CCL5 and GM-CSF in HIE children were (83.41±10.95) ng/L and (52.27±11.71) pg/mL, respectively, which were significantly different from those in healthy group (P< 0.05).With the aggravation of the disease, the expression level of CCL5 in serum of HIE children increased, while the expression level of GM-CSF decreased, and the difference was statistically significant (P<0.05). Serum CCL5 level was negatively correlated with NBNA score (r=- 0.318, P<0.05), GM-CSF level was positively correlated with NBNA score (r=0.338, P<0.05), and CCL5 level was negatively correlated with GM-CSF level (r=-0.359, P<0.05). One year follow-up showed that the GDS score of children with high expression of CCL5 was lower than that of children with low expression of CCL5, and the GDS score of children with high expression of GM-CSF was higher than that of children with low expression of GM-CSF (P<0.05). Conclusions The expression of serum CCL5 is up-regulated and that of GM-CSF is down-regulated in HIE. The levels of CCL5 and GM-CSF are related to the prognosis of HIE children, which may be used as potential markers to evaluate the prognosis of HIE children.
查看全文   查看/发表评论  下载PDF阅读器
关闭