| Objective To analyze the expression of heat shock protein 60 (HSP60) and thymidine kinase 1 (TK1) in cervical cancer tissues, cervical intraepithelial neoplasia (CIN) tissues and normal cervical tissues. Methods A total of 42 patients with cervical cancer (cervical cancer group), 40 patients with CIN (CIN group), and 40 healthy individuals (normal cervix group) were selected from Zhumadian Central Hospital between May 2013 and May 2014. The expression of HSP60 and TK1 protein in each group was detected by SP immunohistochemical method (expressed as optical density). The expression of HSP60 and TK1 in the three groups was compared. 1~72 months of follow-up was conducted. With the median expression level of HSP60 protein in cervical cancer tissue (3.48) as the cut-off point, the patients were divided into two subgroups (HSP60 < 3.48 group, HSP60 > 3.48 group). With the median expression level of TKI protein in cervical cancer tissue (3.60) as the cut-off point, the patients were divided into two subgroups (TK1<3.60 group, TKI>3.60 group). The 5-year survival rates of different subgroups were recorded. According to the presence or absence of recurrence, they were divided into recurrence group and non-recurrence group. Risk factors affecting the prognosis of cervical cancer patients were screened by COX regression analysis. The correlation between HSP60 and TKI was discussed through Pearson correlation analysis. Results The expression levels of HSP60 and TKI protein in cervical cancer group were higher than those in CIN group and normal cervix group (P<0.05). There were statistically significant differences in the expression of HSP60 and TKI proteins between cervical cancer patients with different differentiation degrees, FIGO staging, invasion depth and lymph node metastasis (P<0.05), but there were no statistically significant differences between cervical cancer patients of different age and pathological types (P>0.05). The 5-year survival rates of HSP60 <3.48 group, HSP60 >3.48 group, TK1<3.60 group, and TKI>3.60 group were 85.71%, 61.90%, 90.48% and 57.14%, respectively (P<0.05). COX regression analysis found that infiltration depth more than 1/2 of the mesenchyme, lymph node metastasis, optical density of HSP60 higher than 3.48, and optical densityTK1 higher than 3.60 were independent risk factors affecting the prognosis of cervical cancer (P<0.05). Pearson correlation analysis showed that there was no significant correlation between HSP60 and TK1 (r=0.058, P>0.05). Conclusions The expression of HSP60 and TKI protein is up-regulated in cervical cancer tissues, and their changes will affect the prognosis of patients with cervical cancer. |
