文章摘要
癫痫患儿血清微小RNA-34a、微小RNA-873表达水平及其临床意义
Expression levels and clinical significance of serum microRNA-34a and microRNA-873 in children with epilepsy
投稿时间：2024-07-11  修订日期：2025-04-26
DOI：
中文关键词: 癫痫  患儿  微小RNA-34a  微小RNA-873  临床诊断
英文关键词: epilepsy  children  micro RNA-34a  micro RNA-873  clinical diagnosis
基金项目:河南省医学科技攻关计划(联合共建)项目(LHGJ20200183)
作者单位邮编
马红彦* 南阳市中心医院 473000
摘要点击次数: 43
全文下载次数: 0
中文摘要:
      目的 探讨血清微小RNA-34a（miR-34a）、微小RNA-873（miR-873）在癫痫患儿血清中表达水平及临床意义。方法 选取本院2021年1月~2023年1月期间收治的110例癫痫患儿为研究组，根据患儿癫痫发作频率将其分为频发组（39例）、持续状态组（28例）和非频发或持续状态组（43例），以同期在我院进行体检的96例健康儿童作为对照组。采用实时荧光定量PCR（qRT-PCR）法检测各组癫痫患儿和对照组血清中miR-34a和miR-873的相对表达水平；血清miR-34a、miR-873对癫痫患儿的临床诊断价值采用受试者工作特征（ROC）曲线进行分析；采用多因素Logistic回归分析血清miR-34a、miR-873对癫痫患儿的影响。结果 与对照组相比，研究组血清中miR-34a表达量（1.70±0.56）显著高于对照组（1.08±0.33），miR-873表达量（0.74±0.18）显著低于对照组（1.01±0.15）（P<0.05）。研究组各组患儿血清中miR-34a表达水平为：频发组>持续状态组>非频发或持续状态组（P<0.05）；miR-873表达水平为：非频发或持续状态组>持续状态组>频发组（P<0.05）。血清miR-34a、miR-873诊断癫痫患儿的曲线下面积（AUC）分别为0.834、0.856，截断值分别为1.412、0.896，灵敏度分别为70.00%、77.27%，特异度分别为87.50%、80.21%，且miR-34a、miR-873联合诊断癫痫患儿的AUC为0.921，灵敏度为74.55%，特异度为94.79%，二者联合进行癫痫患儿临床诊断具有更高的价值。多因素分析结果显示，miR-34a是癫痫患儿的独立危险因素；miR-873是癫痫患儿的独立保护因素。结论 miR-34a在癫痫患儿血清中高表达，miR-873在癫痫患儿血清中低表达。miR-34a和miR-873联合应用表现出更高的临床诊断价值。
英文摘要:
      Objective To investigate the expression levels and clinical significance of serum microRNA-34a (miR-34a) and microRNA-873 (miR-873) in children with epilepsy. Methods From January 2021 to January 2023, 110 epileptic children accepted by our hospital were collected as the study group. According to the frequency of seizure, they were grouped into frequent group (39 cases), persistent group (28 cases) and non frequent or persistent group (43 cases). 96 healthy children who underwent physical examination were taken as the control group. real-time fluorescence quantitative PCR (qRT-PCR) method was applied to detect the relative expression levels of miR-34a and miR-873 in the serum of epileptic children and control groups in each group; the clinical diagnostic value of serum miR-34a and miR-873 in children with epilepsy was analyzed using receiver operating characteristic (ROC) curves; multivariate Logistic regression was applied to analyze the impacts of serum miR-34a and miR-873 on children with epilepsy. Results Compared with the control group, the expression level of miR-34a (1.70±0.56) in the serum of the study group was obviously higher than that of the control group(1.08±0.33), while the expression level of miR-873 (0.74±0.18) was obviously lower than that of the control group(1.01±0.15) (P<0.05). The expression level of miR-34a in the serum of children in the study group showed: frequent group>persistent group>non frequent or persistent group (P<0.05); the expression level of miR-873 showed: non frequent or persistent group>persistent group>frequent group (P<0.05). The area under the curve (AUC) of serum miR-34a and miR-873 for diagnosing epilepsy in children was 0.834 and 0.856, respectively, with truncation value of 1.412 and 0.896, sensitivity of 70.00% and 77.27%, and specificity of 87.50% and 80.21%, respectively, moreover, the AUC of miR-34a combined miR-873 for diagnosing epilepsy in children was 0.921, sensitivity of 74.55%, and specificity of 94.79%, the combination of the two had higher value in clinical diagnosis of epilepsy in children. The results of multivariate analysis showed that miR-34a was an independent risk factor for epilepsy in children; miR-873 was an independent protective factor for children with epilepsy. Conclusion MiR-34a is highly expressed in the serum of children with epilepsy, while miR-873 is low expressed in the serum of children with epilepsy. The combination of miR-34a and miR-873 demonstrates higher clinical diagnostic value.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭