| Objective To investigate the expression of miR-378a-5p in subcutaneous adipose tissue of psoriasis and its impact on the pathogenesis of psoriasis. Methods A psoriasis mouse model was constructed from SPF grade 4~6 week old female C57BL/6 mice, where the model group was psoriasis mice and the control group was C57BL/6 mice with petroleum jelly cream. Hematoxylin eosin (HE) staining was used to observe the pathological changes in skin tissue. Real time fluorescence quantitative polymerase chain reaction (qPCR) was used to detect the mRNA levels of miR-378a-5p and Fas apoptosis inhibitory molecule (FAIM) in subcutaneous adipose tissue, and Western blot (WB) was used to detect the protein level of FAIM. Adipocytes were isolated for subsequent experiments. Dual-luciferase reporter assay, qPCR, and WB were used to validate the targeting relationship between miR-378a-5p and FAIM. Plasmids for overexpression and knockdown of miR-378a-5p, as well as FAIM knockdown, were constructed. After transfection into adipocytes, enzyme-linked immunosorbent assay (ELISA) was employed to measure the levels of inflammatory factors--tumor necrosis factor α (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6) in culture medium. Results The skin tissue of the model group mice showed obvious pathological morphology of psoriasis. In the adipose tissue of psoriasis mouse model, the mRNA level of miR-378a-5p was upregulated, while the mRNA and protein levels of FAIM were downregulated, and the differences were statistically significant (P<0.05). Dual-luciferase reporter assay, qPCR, and WB confirmed that FAIM was a downstream target of miR-378a-5p. Compared to the NC mimics group, the miR-378a-5p mimics group showed a significant increase in the release of inflammatory factors TNF- α, IL-1, and IL-6 from adipocytes (P<0.05). Compared to the NC inhibitors group, the miR-378a-5p inhibitors group showed a significant decrease in the release of TNF- α, IL-1, and IL-6 (P<0.05). Compared to the control group, the FAIM inhibitors group showed a significant increase in the release of TNF-α, IL-1, and IL-6 from adipocytes, which could be reversed by miR-378a-5p inhibitors+ FAIM inhibitors (P<0.05). Conclusion MiR-378a-5p silences FAIM, promoting the release of pro-inflammatory factors TNF-α, IL-1, and IL- 6 from subcutaneous adipocytes in psoriasis. |
