| Objective To investigate the expression levels of long non-coding RNA (LncRNA) surfactant associated 1 pseudogene (SFTA1P) and ubiquitin-conjugating enzyme E2R2 antisense RNA 1(UBE2R2-AS1) in the serum of cervical cancer (CC) patients and their correlation with prognosis Methods A total of 108 CC patients admitted to Handan Maternal and Child Health Hospital between April 2018 and April 2021 were selected as the CC group, and 100 healthy individuals during the same period were selected as the health group. Real time fluorescence quantitative PCR (qRT-PCR) was used to detect the levels of serum LncRNA SFTA1P and LncRNA UBE2R2-AS1 in each group; receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic value of serum LncRNA SFTA1P and LncRNA UBE2R2- AS1 for CC; Kaplan-Meier method was used to analyze the relationship between the expression levels of serum LncRNA SFTA1P and LncRNA UBE2R2-AS1 and the prognosis of CC patients; Cox regression was applied to analyze the influencing factors of prognosis in CC patients. Results Compared with the health group, the expression level of serum LncRNA SFTA1P in CC patients increased (P<0.05), the expression level of LncRNA UBE2R2-AS1 was reduced (P<0.05), and their expression levels were related to FIGO staging, lymph node metastasis, and depth of muscle layer infiltration (P<0.05). The area under the curve for diagnosing CC by serum LncRNA SFTA1P and LncRNA UBE2R2- AS1 alone and in combination was 0.863, 0.817, and 0.915, respectively, and the combined diagnosis was superior to individual diagnoses (Zcombination - LncRNA SFTA1P=3.057, P=0.002, Zcombination-LncRNA UBE2R2-AS1=3.564, P<0.001). The 3-year survival rate in the serum LncRNA SFTA1P high expression group was lower than that in the low expression group (64.91% vs 80.39%, χ2=4.443, P=0.035), and the 3-year survival rate in the LncRNA UBE2R2-AS1 high expression group was higher than that in the low expression group (82.00% vs 63.79%, χ2=5.938, P=0.015). FIGO staging of stages IIb~IIIc, lymph node metastasis, muscle layer infiltration depth ≥ 1/2, the increased expression level of LncRNA SFTA1P and the decreased expression level of LncRNA UBE2R2-AS1 were both risk factors for the prognosis of CC patients (P<0.05). Conclusion The expression of LncRNA SFTA1P in the serum of CC patients is upregulated, the expression of LncRNA UBE2R2-AS1 is downregulated, and their levels are related to tumor FIGO staging, lymph node metastasis, depth of muscle layer infiltration, and are closely related to prognostic survival. |
