| Objective To investigate the relationship between the expression of long non-coding RNA nuclear enriched abundant transcript 1 (LncRNA-NEAT1) and microRNA-93-5p (miR-93-5p) in the serum of epileptic children and EEG abnormalities and prognosis. Methods A total of 99 children with epilepsy who were treated in the Department of Pediatrics, Handan Central Hospital from May 2019 to May 2021 were regarded as the observation group. According to the severity of abnormal EEG, there were 37 cases recruited in the mild abnormal EEG group, 22 cases in the moderate abnormal EEG group, and 40 cases in the severe abnormal EEG group. Patients were followed up for two years, and were divided into poor prognosis group (38 cases) and good prognosis group (61 cases) according to the follow-up results. Another 99 healthy children who underwent health examinations in our hospital and matched general information with epilepsy children were collected as the control group. qRT-PCR was applied to detect the expression levels of serum LncRNA NEAT1 and miR-93-5p in all subjects. Spearman method was used to analyze the correlation between LncRNA-NEAT1 and miR-93-5p and the severity of EEG abnormalities. Logistic regression was applied to analyze the influencing factors of severe EEG abnormalities and poor prognosis in children with epilepsy. Results The serum LncRNA NEAT1 level in the observation group was obviously higher than that in the control group, and miR-93-5p level was obviously lower than that in the control group (P<0.05); the serum levels of LncRNA-NEAT1 and miR-93-5p in epileptic children with different degrees of EEG abnormalities were statistically different (P<0.05); LncRNA-NEAT1 was positively correlated with the severity of EEG abnormalities (r=0.801, P<0.05), while miR-93-5p was negatively correlated with the severity of EEG abnormalities (r=-0.661, P<0.05); Long course of disease, seizure frequency > 1 time/day, and high expression of LncRNA NEAT1 were all risk factors for severe abnormalities in electroencephalogram in children with epilepsy (P<0.05), while high expression of miR-93-5p was a protective factor (P<0.05); the serum LncRNA NEAT1 level in the poor prognosis group was obviously higher than that in the good prognosis group, and the miR-93-5p level was obviously lower than that in the good prognosis group (P<0.05); high expression of LncRNA-NEAT1 was a risk factor for poor prognosis in children with epilepsy, while high expression of miR-93-5p was a protective factor (P<0.05). Conclusion The level of serum LncRNA-NEAT1 in children with epilepsy increases and is positively correlated with the severity of EEG abnormality, which is a risk factor for poor prognosis, while the level of miR-93-5p decreases and is negatively correlated with the severity of EEG abnormality, which is a protective factor for poor prognosis. |
