| Objective To investigate the expression levels and clinical significance of serum microRNA-34a（miR-34a） and microRNA-873（miR-873） in children with epilepsy. Methods According to the frequency of epileptic seizures in children, 110 children with epilepsy admitted to Nanyang Central Hospital from January 2021 to January 2023（study group） were divided into a non frequent or persistent state group（43 cases）, a persistent state group（28 cases）, and a frequent group（39 cases）. During the same period, 96 healthy children who underwent physical examination at Nanyang Central Hospital were selected as the control group. Real time fluorescence quantitative polymerase chain reaction was used to detect the levels of miR-34a and miR-873 in serum; The clinical diagnostic value of serum miR-34a and miR-873 in children with epilepsy was analyzed using receiver operating characteristic（ROC） curves; Using multiple logistic regression analysis to investigate the influencing factors of epilepsy in pediatric patients. Results Compared with the control group, the expression level of miR-34a in the serum of the study group was higher than that of the control group, and the expression level of miR-873 was lower than that of the control group（P＜0.05）. The serum miR-34a levels in children with epilepsy in the frequent group, persistent group, and non frequent or persistent group decreased sequentially, while miR-873 levels increased sequentially, with statistically significant differences（P＜0.05）. The area under the curve（AUC） of serum miR-34a, miR-873, and their combined diagnosis in children with epilepsy were 0.834, 0.856, and 0.921, respectively. The sensitivities were 70.00%, 77.27%, and 74.55%, and the specificities were 87.50%, 80.21%, and 94.79%, respectively. The results of multivariate analysis showed that high expression of miR-34a is an independent risk factor for children with epilepsy; High expression of miR-873 is an independent protective factor in children with epilepsy. Conclusion MiR-34a is highly expressed in the serum of children with epilepsy, while miR-873 is lowly expressed in the serum of children with epilepsy. The two are closely related to the frequency of seizures and diagnosis of epilepsy. |
