| Objective To analyze the expression of long non-coding RNA (Lnc RNA) X chromosome inactivation specific transcript(XIST) and micro RNA-101-3p (mi R-101-3p) in peripheral blood mononuclear cells of patients with systemic lupus erythematosus and their clinical application value.Methods A total of 180 patients with systemic lupus erythematosus who were treated in Xinxiang Central Hospital from April 2019 to October 2021 were selected as the observation group, and 180 healthy people in the same period were recruited as the con-trol group.According to SLEDAI score, the patients were divided into stable stage group (80 cases) and active stage group (100 cases).The rela-tive expression levels of Lnc RNA XIST and mi R-101-3p in peripheral blood mononuclear cells of patients were detected by real-time fluores-cent quantitative PCR (q RT-PCR).The correlation of Lnc RNA XIST with mi R-101-3p and correlation of the two with SLEDAI scores were analyzed, logistic regression analysis was carried out on the factors influencing SLE, receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of the expression of Lnc RNA XIST and mi R-101-3p for systemic lupus erythematosus.Results Compared with the control group, the level of Lnc RNA XIST in peripheral blood mononuclear cells of systemic lupus erythematosus patients in the observation group was obviously higher, the level of mi R-101-3p was obviously lower, and the differences were statistically significant(P<0.05);the level of Lnc RNA XIST in peripheral blood mononuclear cells of patients with systemic lupus erythematosus in the active phase group was markedly higher than that in the stable phase group, while the level of mi R-101-3p was apparently lower, and the differences were statistically signifi-cant (P<0.05), there was a negative correlation between Lnc RNA XIST and mi R-101-3p levels in peripheral blood monocytes of patients (r=-0.410, P<0.05), Lnc RNA XIST level was positively correlated with SLEDAI score (r=0.425, P<0.05), and mi R-101-3p level was negatively correlated with SLEDAI score (r=-0.454, P<0.05), logistic regression analysis showed that Lnc RNA XIST was a risk factor for systemic lupus erythematosus (P<0.05), and mi R-101-3p was a protective factor for systemic lupus erythematosus (P<0.05), the area under the ROC curve of Lnc RNA XIST and mi R-101-3p in the combined diagnosis of systemic lupus erythematosus was 0.960, which was superior to their respective diagnosis alone (Zcombination-LncRNA XIST=3.268, P=0.001, Zcombination-miR-101-3p=2.584, P=0.005).Conclusion The level of Lnc RNA XIST in peripheral blood mononuclear cells of patients with systemic lupus erythematosus increases, and the level of mi R-101-3p decreases;they are related to the disease activity of systemic lupus erythematosus and have certain diagnostic value for systemic lupus erythematosus. |
