文章摘要
竹红素A联合LED红光照射通过YAP途径抑制瘢痕疙瘩成纤维细胞增殖
Hypocrellin A combined with LED red light irradiation inhibits the proliferation of keloid fibroblasts through the YAP pathway
投稿时间:2023-06-27  
DOI:10.3969/j.issn.1000-0399.2024.02.002
中文关键词: 瘢痕疙瘩  成纤维细胞  竹红素A  增殖  氧化应激  Yes相关蛋白
英文关键词: Keloid  Fibroblasts  Hypocrellin A  Proliferation  Oxidative stress  Yes associated proteins
基金项目:河北省中医药管理局科研计划项目(编号:2023105)
作者单位E-mail
陈雷刚 075000 河北张家口 河北北方学院附属第一医院皮肤科 ZJKanguozhi@163.com 
任慧敏 075000 河北张家口 河北北方学院附属第一医院急诊科  
吴远慧 075000 河北张家口 河北北方学院附属第一医院皮肤科  
安国芝 075000 河北张家口 河北北方学院附属第一医院皮肤科  
景晓蕾 075000 河北张家口 河北北方学院研究生院  
赵同心 075000 河北张家口 河北北方学院研究生院  
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中文摘要:
      目的 探究hypocrellin A(HA)联合LED红光照射通过Yes相关蛋白(YAP)途径抑制瘢痕疙瘩成纤维细胞(KFs)增殖的作用及机制。方法 原代培养KFs并进行传代,第3代KFs分为对照组、H组(1.0μmol/L HA处理)、L组(3 J/cm2红光照射)、H+L组、阴性对照(NC)质粒组、NC质粒+H+L组、YAP质粒+H+L组、检测细胞增殖的OD450水平及BrdU阳性率,氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量,YAP、β-连环蛋白(β-catenin)的表达水平。结果 H组、L组、H+L组KFs的OD450、BrdU阳性率、SOD及GSH-Px的含量、YAP及β-catenin的表达水平均低于对照组,MDA的含量高于对照组(P<0.05),且H+L组上述指标的变化较H组、L组更明显(P<0.05);YAP质粒+H+L组KFs的OD450、BrdU阳性率、SOD及GSH-Px的含量、YAP及β-catenin的表达水平均高于NC质粒+H+L组,MDA的含量低于质粒+H+L组(P<0.05)。结论 HA联合LED红光照射通过抑制YAP/β-catenin途径抑制KFs增殖、激活KFs氧化应激。
英文摘要:
      Objective To investigate the effect of Hypocrellin A (HA) combined with LED red light irradiation on inhibiting the proliferation of keloid fibroblasts (KFs) through the Yes associated protein (YAP) pathway and explore the underlying mechanism.Method KFs were primarily cultured and passaged.The 3rd generation KFs were divided into the control group and H group (1.0μ Mol/L HA treatment),L group (3J/cm2 red light irradiation),H+L group,negative control (NC) plasmid group,NC plasmid+H+L group,YAP plasmid+H+L group.Cell proliferation OD450 level and BrdU positive rate,content of oxidative stress indicators malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH Px),expression of YAP and β-catenin were detected.Results OD450,BrdU positive rate,SOD and GSH-Px content,YAP and β-catenin expression levels of H group,L group,H+L group were lower than those of the control group,and the content of MDA was higher than that of the control group (P<0.05).The changes of the above indicators in the H+L group were more significant than those in the H and L groups (P<0.05).OD450,BrdU positive rate,SOD and GSH-Px content,YAP and β-catenin expression levels of YAP plasmid+ H+L group were higher than those of the NC plasmid+H+L group,and the content of MDA was lower than that of the NC plasmid+H+L group (P <0.05).Conclusion HA combined with LED red light irradiation inhibits the proliferation of and activates oxidative stress of KFs via inhibiting YAP/β-catenin pathway.
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