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| 结核菌快速培养法与γ-干扰素释放试验在结核病诊断中的应用价值 |
| The clinical values of TB rapid culture method and γ-interferon release assay in diagnosis of tuberculosis |
| 投稿时间：2015-01-11 修订日期：2015-04-15 |
| DOI：10.3969/j.issn.1000-0399.2015.05.027 |
| 中文关键词: 结核菌快速培养法 γ-干扰素释放试验 结核病 |
| 英文关键词: TB rapid culture γ-interferon release assay Tuberculosis |
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| 摘要点击次数: 1997 |
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| 中文摘要: |
| 目的 探讨结核菌快速培养法与γ-干扰素释放试验在结核病诊断中的应用价值。 方法 选择2013年11月至2014年7月在我院进行检查的与结核病密切接触者180例,均进行结核菌快速培养法与γ-干扰素释放试验检测结核杆菌,以结核分枝杆菌培养结果为对照,分析两种检测方法结核杆菌阳性检出率的敏感性及特异性,并分析导致结核菌快速培养法误诊的危险因素。 结果 结核菌快速培养法和γ-干扰素释放试验结核菌检出阳性率分别是13.3% 和16.1%,两者比较差异无统计学意义(P>0.05)。以结核分枝杆菌培养结果为金标准,结核菌快速培养法和γ-干扰素释放试验的诊断敏感性分别为70.0%和96.7%,特异性分别为98.0%和 100.0%。γ-干扰素释放试验的诊断敏感性明显高于结核菌快速培养法,差异有统计学意义(P<0.05)。logistic多元回归分析表明年龄和既往结核史是导致结核菌快速培养法误诊的主要因素 (P<0.05)。 结论 相对于结核菌快速培养法,γ-干扰素释放试验结核病诊断的敏感性更高。 |
| 英文摘要: |
| Objective To investigate the clinical values of TB rapid culture method and γ-interferon release assay in the diagnosis of tuberculosis. Methods A total of 180 close contact cases with tuberculosis from November 2013 to July 2014 in our hospital were selected and given the rapid TB culture and γ-interferon release assay, then the risk factors for misdiagnosis were analysed. Results The detection positive rate of the TB rapid culture method and γ-interferon release assay was respectively 13.3% and 16.1%, and the difference had no statistical significance (P>0.05). With mycobacterium tuberculosis sputum culture results as the gold standard, the sensitivity of the TB rapid culture method and γ-interferon release assay was respectively 70.0% and 96.7%, and the specificity was 96.7% and 100.0%. The diagnostic sensitivity of the γ-interferon release assay was significantly higher than that of the rapid culture method (P<0.05). Logistic regression analysis showed that the factors leading to TB rapid culture method misdiagnosis were mainly age and previous history of tuberculosis (P<0.05). Conclusion Compared with TB rapid culture, γ-interferon release assay in the diagnosis of tuberculosis in clinical applications has better detection rate and higher sensitivity. |
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