|
| 大鼠炎症牙髓中钠离子通道Nav1.7的表达 |
| Expression of voltage gated sodium channel Nav1.7 in rat pulpitis |
| 投稿时间：2014-11-18 修订日期：2015-03-20 |
| DOI：10.3969/j.issn.1000-0399.2015.06.001 |
| 中文关键词: 大鼠 炎症 牙髓炎 Nav1.7 |
| 英文关键词: Rats Inflammation Pulpitis Nav1.7 |
| 基金项目:安徽医科大学校基金课题(项目编号:2012xkj022) |
|
| 摘要点击次数: 1456 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨大鼠炎症牙髓中钠离子通道Nav1.7的表达。方法 60只SD大鼠分为正常组(A组)和炎症组, 炎症组中, 封脂多糖(LPS)1 d为B组、3 d为C组、5 d为D组。炎症组通过置入LPS诱导产生牙髓炎。通过HE染色观察各组大鼠牙髓组织病理学改变, 通过免疫组化和ELISA检测各组大鼠牙髓中Nav1.7的表达。结果 各组牙髓组织病理学发生改变, 各组牙髓切片中均有Nav1.7的表达。ELISA结果显示, 4组牙髓中Nav1.7的表达量分别为(3.38±0.10)、(3.67±0.15)、(4.04±0.14)和(4.25±0.11)μg/L, 各组间差异有统计学意义(P<0.05)。结论 Nav1.7的表达增高可能与牙髓炎症具有潜在关联性。 |
| 英文摘要: |
| Objective To investigate the expression of voltage gated sodium channel Nav1.7 in rat pulpitis. Sixty rats were randomly divided into a nontreatment group(A)and three inflammation groups: day1(B), day3(C) and day5(D), and pulpitis was induced by creating pulp exposures to LPS in rat incisions. Histopathological changes and the expression of Nav1.7 were observed by Hematoxylin-eosin staining, immunohistochemistry and ELISA(Enzyme-linked immunosorbent assay) in each team. Results Histopathological changes were revealed in each team, while Nav1.7 was also expressed. ELISA results revealed that the level of Nav1.7 in control pulp tissue was (3.38±0.10) μg/L, in painful pulp tissue of 1d group was(3.67±0.15)μg/L, in painful pulp tissue of 3d group was(4.04±0.14)μg/L and in painful pulp tissue of 5d group was(4.25±0.11)μg/L(P<0.05). Conclusion The relationship between dental pain and Nav1.7 expression level maybe exist. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
