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| 脂多糖对正常人肝内胆管上皮细胞TLR4-NF-кB通路的影响 |
| Effect of LPS on TLR4-NF-кB pathway in human intrahepatic biliary epithelial cells |
| 投稿时间：2015-01-20 修订日期：2015-03-19 |
| DOI：10.3969/j.issn.1000-0399.2015.07.034 |
| 中文关键词: 人正常肝内胆管上皮细胞 脂多糖 Toll样受体4 核转录因子-кB |
| 英文关键词: Human intrahepatic biliary epithelial cells Lipopolysaccharides Toll-like receptor 4 Nuclear factor-κB |
| 基金项目:安徽省年度重点科研项目(1301043034) |
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| 中文摘要: |
| 目的 探讨脂多糖(LPS)对正常人肝内胆管上皮细胞(HiBECs)中TLR4-NF-кB通路的影响。方法 HiBECs常规细胞培养后,加入不同浓度的LPS(0.1、1、4、8、10 μg/mL),刺激不同时间(3、6、9、12、24 h),用Realtime RT-PCR分析细胞内TLR4和NF-κB mRNA表达水平。结果 LPS刺激HiBECs后TLR4和NF-κB mRNA表达水平均增加,HiBECs在受到LPS(4 μg/mL)刺激后TLR4和NF-κB mRNA表达水平随时间延长而增加,于6小时达高峰,之后下降。以相同时间(6 h)刺激HiBECs后,TLR4和NF-κB mRNA表达水平随LPS浓度增高而增加,LPS浓度为4 μg/mL时达高峰,之后下降。结论 LPS可以激活HiBECs中的TLR4-NF-кB通路,并且有时效和量效依赖关系。 |
| 英文摘要: |
| Objective To investigate the effect of lipopolysaccharides (LPS) on TLR4-NF-кB pathway in human intrahepatic biliary epithelial cells (HiBECs) in vitro.Methods HiBECs were cultured and divided into normal control group (NC) and LPS group. LPS group was treated with LPS at different time (3 h, 6 h, 9 h, 12 h, 24 h)and different concentrations (0.1, 1, 4, 8, 10 μg/mL). The expression of TLR4 and NF-кB was assessed by realtime-PCR and Western blot.Results LPS upregulated the expression of TLR4 and NF-кB in HiBECs. The TLR4 and NF-κB mRNA transcription was increased at different time of LPS stimulation, and reached its peak on 6 h and then gradually decreased. Simultaneously, the TLR4 and NF-κB mRNA expression level was increased in a dose-dependent manner,and reached its peak when used at 4 μg/mL LPS and then decreased.Conclusion LPS activates TLR4-NF-кB signaling in HiBECs in a time-and dose-dependent manner. |
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