|
| 雌激素抑制大鼠肝癌肺转移机制研究 |
| The mechanism study of inhibition of hepatic tumor lung metastasis by estrogen in a rat model |
| 投稿时间：2015-05-19 修订日期：2015-07-03 |
| DOI：10.3969/j.issn.1000-0399.2015.12.001 |
| 中文关键词: 肝细胞肝癌 肝癌肺转移 雌激素 炎症因子 |
| 英文关键词: Hepatocellular carcinoma Lung metastasis Estrogen Inflammatory factor |
| 基金项目:国家自然科学基金项目(项目编号:30972893) |
|
| 摘要点击次数: 2052 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨雌激素对大鼠肝癌肺转移形成的影响及其可能的机制.方法 将雌性大鼠随机分为切除卵巢组、对照组、假手术组和去卵巢后加雌激素组，建立大鼠肝癌模型.诱癌20周后观察肿瘤发生、肺转移的情况;免疫组织化学法检测4组成瘤大鼠肝癌组织中雌激素受体α(ERα)、磷酸化雌激素受体α(P-ERα)、肝细胞生长因子(HGF)和白介素6(IL-6)的表达;半定量逆转录聚合酶链反应法检测ERα、HGF及IL-6的mRNA水平.结果 切除卵巢组大鼠肝癌肺转移发生率明显高于其他3组(P<0.05);大鼠肝癌肺转移发生率越高，肝癌组织中HGF、IL-6的蛋白及分子水平表达越高(P<0.05),而ERα的蛋白及分子水平表达越低(P<0.05).结论 雌激素可能通过下调HGF和IL-6的表达来抑制肝癌肺转移的发生. |
| 英文摘要: |
| Objective To determine whether and how estrogen reduced lung metastasis of HCC in a rat model. Methods We conducted a rat HCC model in female rats induced by diethylnitrosamine (DEN) and N-nitrosomorpholine (NMOR), and rats were distributed into four groups: ovariectomy, sham operation, ovariectomy followed by 30 μg/(kg·d) body weight/day 17α-Ethynylestradiol (EE2) supplementation, sexually intact controls. Results Here we showed that, 60% lung metastasis was observed in the rats of ovariectomy group, whereas obviously lower lung metastasis was found in the rats of other three groups. Furthermore, the higher expression of interleukin-6 (IL-6), hepatocyte growth factor (HGF) and the lower expression of estrogen receptor α(ERα), the higher lung metastasis of HCC we founded. Conclusion Estrogen may inhibit hepatic tumor lung metastasis by suppression of HGF and IL-6 production. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
