|
| MicroRNA-129-2靶向调控SOX4抑制食管癌细胞增殖与侵袭转移的研究 |
| miR-129-2 inhibits proliferation and invasion of esophageal carcinoma cells by targeting of SOX4 |
| 投稿时间：2015-11-05 修订日期：2015-11-28 |
| DOI：10.3969/j.issn.1000-0399.2015.12.002 |
| 中文关键词: miR-129-2 SOX4 食管癌 增殖 侵袭 |
| 英文关键词: MicroRNA-129-2 SOX4 Esophageal carcinoma Proliferation Invasion |
| 基金项目:国家自然科学基金(项目编号:81460460);广西壮族自治区科技厅青年基金(项目编号:2014GXNSFBA118144);广西医科大学青年基金(项目编号:GXMUYSF201219) |
|
| 摘要点击次数: 2367 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨MicroRNA-129-2(miR-129-2)在体内外抑制食管癌细胞增殖和侵袭转移的作用及可能机制,为食管癌的预后评估及靶向治疗提供新的思路.方法 将miR-129-2 mimics及miR-129-2 mimics NC基因序列分别转染到食管癌NMC109细胞中,并设为miR-129-2 mimics高表达组和miR-129-2 mimics阴性对照组(miR-129-2 mimics NC组),将非转染的NMC109细胞设为空白对照组.体外实验:运用MTT法检测3组细胞的增殖能力、Transwell法检测细胞侵袭能力、蛋白印迹法检测SOX4蛋白表达.采用裸鼠体内移植瘤形成实验检测3组细胞在体内环境下的增殖能力.结果 miR-129-2 mimics组的食管癌细胞增殖活性及侵袭能力较miR-129-2 mimics NC组及空白对照组明显减弱(P<0.001),而空白对照组与miR-129-2 mimics NC组食管癌细胞增殖性及侵袭性无明显差异(P>0.05);miR-129-2 mimics组的食管癌细胞中,SOX4的表达下调(P<0.001);miR-129-2 mimics组裸鼠移植瘤体积及重量明显减小(P<0.001),而miR-129-2mimics NC组及空白对照组无明显差异(P>0.05).结论 miR-129-2具有抑制食管癌细胞NMC109增殖和侵袭转移的作用,其作用机制可能与靶向下调SOX4基因表达有关. |
| 英文摘要: |
| Objective To explore the effect of MicroRNA-129-2 (miR-129-2) in suppressing the proliferation and invasion of esophageal carcinoma cells in vivo and in vitro and the possible mechanism, so as to provide new thoughts for the prognosis evaluation and targeted therapy of the disease. Methods Two groups of stable-transfected cells including miR-129-2 mimics group and miR-129-2mimics NC group. A blank control group were constructed. Detecting the proliferation and invasion of the three groups of cells by MTT Assays and invasion assay in vitro, SOX4 protein expression was detected in each group of cells using Western blot method, xenograft experiments in vivo were used in investigating the effect and mechanism of miR-129-2 in proliferation and invasion of esophageal carcinoma cells. Results The esophageal carcinoma cells' proliferation and invasion activity decreased significantly after transfected MicroRNA-129-2 mimics(P<0.001). The esophageal carcinoma cells transfected MicroRNA-129-2 mimics exhibited down expression of SOX4(P<0.001). Xenograft experiments showed that miR-129-2 led a significant decrease in tumor volume and weight (P<0.001), whereas the miR-129-2 mimics NC and blank control group indicated no effect(P>0.05). Conclusion miR-129-2 may suppress the proliferation, invasion and metastasis of NMC109 esophagus carcinoma cells by targeting down-regulation of SOX4 expression. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
