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| TopoⅡα和Ki67在浸润性乳腺癌中的表达及其临床意义 |
| Expression and clinical significance of TopoⅡα and Ki67 in invasive breast cancer tissues |
| 投稿时间：2015-09-20 |
| DOI：10.3969/j.issn.1000-0399.2016.01.005 |
| 中文关键词: 浸润性乳腺癌 拓扑异构酶Ⅱα 增殖相关核抗原Ki67 免疫组化 |
| 英文关键词: Invasive breast cancer Topoisomerase Ⅱα Ki67 Immunohistochemistry |
| 基金项目:安徽省科学技术厅科技计划项目(项目编号:12010402124) |
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| 中文摘要: |
| 目的 探讨TopoⅡα、Ki67在浸润性乳腺癌中的表达及其与乳腺癌患者一般临床病理特征之间的关系。方法 回顾性分析接受手术治疗的78例乳腺癌病例资料,采用免疫组化方法检测TopoⅡα及Ki67的表达情况,并分析两种蛋白与患者一般临床病理特征的关系。结果 TopoⅡα蛋白阳性率48.72%(38/78),Ki67蛋白表达阳性率66.67%(52/78),TopoⅡα与Ki67均阳性表达率38.46%(30/78)。TopoⅡα与乳腺癌患者的年龄、肿块大小、雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(HER-2)均无明显相关(P>0.05),与Ki67的表达及淋巴结转移组数有关(P<0.05)。Ki67的表达与乳腺癌患者的年龄、肿块大小、ER、HER-2均无明显相关(P>0.05),与淋巴结转移数及孕激素受体PR相关(P<0.05)。TopoⅡα、Ki67共表达与肿瘤组织大小及淋巴结转移组数相关(P<0.05),而与年龄、ER、PR等无关(P>0.05)。多因素logistic回归分析显示,淋巴结转移组数(P=0.007,OR=1.781,95%CI:1.175~2.700)是TopoⅡα阳性表达的独立预测因子,淋巴结转移组数(P=0.032,OR=1.730,95%CI:1.049~2.855)及PR蛋白表达状态(P=0.000,OR=0.126,95%CI:0.039~0.402)是Ki-67阳性表达的独立预测因子,淋巴结转移组数(P=0.005,OR=1.819,95%CI:1.198~2.762)是TopoⅡα及Ki-67共表达的独立预测因子。结论 TopoⅡα与Ki67均可作为可反映肿瘤细胞的增殖活性的指标,联合检测两者表达水平可以为乳腺癌预后判断提供参考。 |
| 英文摘要: |
| Objective To analyze the correlation between the expression of topoisomeraseⅡalpha and Ki67 with the clinical and pathological characteristics in invasive breast cancer.Methods The 78 breast cancer patients who accepted surgical treatment were retrospectively studied and tested by immunohistochemistry(IHC) staining.Compare the relationship between the expression of topoisomeraseⅡα and Ki67 with the clinical and pathological characteristics by statistics analyze.Results The results showed that the positive expression of TopoⅡα was 66.67% (52/78), Ki67 positive rate was 48.71% (38/78), TopoⅡα and Ki67 co-expression rate was 38.46%(30/78).There were no statistical differences between TopoⅡα and age, tumor size,ER,PR or HER-2(P>0.05).Statistically significant differences and positive correlations in TopoⅡα expression between the ones with lymph node metastases or not(P<0.05).Statistical differences and positive correlations were noted in TopoⅡα expression between Ki67(+) and Ki67(-)(P<0.05).It was showed that there is correlation between the expression of ki67 with lymph node metastasis(P<0.05).Statistically significant differences in the presence of PR(+)and PR (-) were noted in the expression rate of Ki67(P<0.05), while there was no direct relationship with age, tumor size, or expression of ER and HER-2(P>0.05).The co-expression of TopoⅡα and Ki67 were found has a correlation with tumor size(P<0.05) and lymph node metastasis(P<0.05), but no relationship were found with age, or expression of ER PR and HER-2(P>0.05).Logistic regression analysis demonstrated that the lymph node metastases or not was independent predictor both of the positive expression of TopoⅡα(P=0.007,OR=1.781,95%CI:1.175~2.700) and the co-expression of TopoⅡα and Ki67(P=0.005,OR=1.819,95%CI:1.198~2.762).It also demonstrated that the lymph node metastases(P=0.032,OR=1.730,95%CI:1.049~2.855) and the positive expression of PR(P=0.000,OR=0.126,95%CI:0.039~0.402) were independent predictors of the positive expression of Ki67.Conclusion Both of TopoⅡα and Ki67 can be used as proliferation markers of tumor cells,the co-expression of TopoⅡα and Ki67 might provides information on the prognosis of breast cancer. |
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