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| N-亚硝基二乙胺(DEN)诱导SD大鼠肝癌模型的研究 |
| Research of N-nitrosodiethylamine induced SD rat model of liver cancer |
| 投稿时间：2015-09-16 |
| DOI：10.3969/j.issn.1000-0399.2016.04.001 |
| 中文关键词: 肝癌模型 N-亚硝基二乙胺 大鼠 |
| 英文关键词: Liver cancer model N-nitrosodiethylamine(DEN) Rat |
| 基金项目:广西医疗卫生适宜技术研究与开发课题(项目编号:S201316-06) |
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| 中文摘要: |
| 目的 建立与人类的肝癌发生发展过程相似的动物模型,以便研究肝癌的发生发展过程。方法 取体质量120~150 g雄性SD大鼠65只,适应环境1周后随机分为实验组(50只)和对照组(15只),实验组饮用含N-亚硝基二乙(DEN)(浓度为76 ppm)(以下简称DEN)的水连续6周后停药,改自由饮水3周,再继续饮用含DEN(76 ppm)的水至20周。对照组常规自由饮水。结果 病理学检查证实DEN可诱发大鼠形成肝癌,14周后成癌率为66.7%(10/15),大鼠肝癌的癌变过程大致可分为肝炎期、肝硬化期和肝癌期三个发展阶段。结论 饲以76 ppm剂量DEN可诱发大鼠肝癌模型的建立,可以为我们研究大鼠肝癌的发生发展过程提供可靠的动物模型。 |
| 英文摘要: |
| Objective To set up an animal model similar to the process of human liver cancer so as to study the tumorigenesis of liver cancer.Methods Sixty-five male SD rats with the weight of 120~150 g were randomly divided into the experimental group(50 rats)and the control group(15 rats)after acclimating for one week. Rats of the experiment group were given DEN(76 ppm)in drinking water for 6 weeks, followed by free drinking water for 3 weeks, and then continued drinking water containing DEN(76 ppm)to 20 weeks. The rats of control group were given free drinking water conventionally.Results Pathological examination confirmed that DEN could induce the formation of liver cancer in rats. After 14 weeks the rate of cancer was about 66.7%(10/15). Rat liver carcinogenesis could be divided into the three stages of hepatitis, cirrhosis and liver cancer.Conclusion Feeding with DEN(76ppm)can induce liver cancer model in rats, which can provide reliable animal model for the study of the carcinogenesis of rat liver cancer. |
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