| Objective To study the effect of Gualou Xiebai Decoction (GLXB-D) on pulmonary fibrosis in rats. Methods the bleomycin induced pulmonary fibrosis in rats,using RIA kit were detected in III and IV type collagen (Col-Ⅲ、Col-Ⅳ),laminin (LN),hyaluronic acid (HA) level by he and Masson staining was detected in the rat lung tissue pathologic changes.Results Compared with the normal group,model group rats body weight significantly reduced,lung index increasing,the difference is statistically significant (P<0.05); compared with the model group,GLXB-D1,GLXB-D2,GLXB-D3 group and prednisone group rats body weight increased,lung index decreased significantly (P<0.05). Compared with the rats in the normal group,the rats in the model group,serum Col-Ⅲ,Col-Ⅳ,LN and HA content increased significantly (P<0.05); compared with the model group,GLXB-D1,GLXB-D2,GLXB-D3 group and prednisone group,the levels of serum III PCol-,col IV and LN and HA were significantly reduced (P<0.05). Conclusion GLXB-D on bleomycin induced pulmonary fibrosis in rats have obvious therapeutic effect,reduce rat serum Col-Ⅲ,Col-Ⅳ,LN,HA content,the effects of extracellular matrix synthesis is the possible mechanism. |
