|
| 白藜芦醇对肾脏缺血再灌注损伤大鼠降低炎症反应及抑制细胞凋亡的作用 |
| Resveratrol protects kidney function by alleviating inflammation and inhibiting apoptosis in rat kidney ischemia-reperfusion injury model |
| 投稿时间：2016-08-15 |
| DOI：10.3969/j.issn.1000-0399.2016.12.002 |
| 中文关键词: 白藜芦醇 缺血再灌注损伤 肾功能 抗炎作用 抑制凋亡 |
| 英文关键词: Resveratrol Ischemia-reperfusion injury Renal function Anti-inflammation Anti-apoptosis |
| 基金项目: |
|
| 摘要点击次数: 1683 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 研究白藜芦醇（RSV）在大鼠肾脏缺血再灌注损伤（IRI）模型中降低炎症反应及抗凋亡作用。方法 将18只SD大鼠随机分成假手术组（Sham组）、IRI组和IRI+RSV组），每组6只，后两组建立IRI组模型，治疗组于术前2天、术前1天和术后第1天给予RSV（0.23μg/kg）灌胃。各组大鼠分别于术后36小时、48小时、第5天采集血标本及肾脏标本，评价肾功能和肾组织病理学损伤，采用原位缺口末端标记法（TUNEL）检测肾小管上皮细胞凋亡情况，采用实时定量反转录聚合酶链式反应（RT-qPCR）检测肾脏组织相关炎性细胞因子水平。结果 在肾脏IRI早期，与IRI组相比，IRI+RSV组大鼠血肌酐和尿素氮的水平明显降低，病理损伤较轻，TUNEL显示肾小管细胞凋亡（阳性细胞）减少，相关炎性细胞因子的表达水平也明显降低。结论 RSV可以通过降低大鼠IRI过程中的炎性反应，并起到抗凋亡的作用，进而发挥对肾脏的保护作用。 |
| 英文摘要: |
| Objective To investigate the protective effects of resveratrol on kidneys exposed to ischemia-reperfusion(IR) injury in early stage in a ratmodel by anti-inflammation and anti-apoptosis.Methods Eighteen SD rats were randomly divided into sham group, IR group and IR+RSV-treated group with six rats in each group. IR model was established in the last two groups by intragastric administration of resveratrol(RSV) (0.23 μg/kg) preoperative 2 d, preoperative 1 d and postoperative 1 d.Blood and renal samples in three groups were collected postoperative 36 h, 48 h and 5 th day for the assessment of renal function and pathological injury of kidney. Renal cell apoptosis was detected by TdT-mediated dUTP nick end labeling (TUNEL) assay. The expression of some related cytokines was detected by RT-qPCR.Results After RVS treatment, the serum creatinine and urea nitrogen level decreasedwith lighter pathological damage compared with IR group. Apoptotic renal cells in IR+RSV group also declined compared with IR group.Conclusion RSV protects renal function probablyby inhibiting cell apoptosis and reducing inflammation. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
