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| 同时下调长链非编码RNA PVT1和MINCR对Raji细胞增殖的影响 |
| Effect of down-regulating long non-coding RNA PVT1 and MINCR on proliferation of Raji cells |
| 投稿时间：2016-10-25 |
| DOI：10.3969/j.issn.1000-0399.2017.05.001 |
| 中文关键词: 长链非编码RNA 人浆细胞瘤转化迁移基因1 MINCR 小干扰RNA 细胞增殖 |
| 英文关键词: Long non-coding RNA Plasmacytoma variant translocation 1 MYC-induced long noncoding RNA Small interfering RNA Cell proliferation |
| 基金项目:广东省科技计划项目（项目编号：2013B021800151，2015A050502029）；国务院侨办重点学科建设基金项目（项目编号：51205002） |
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| 中文摘要: |
| 目的 探讨同时下调长链非编码RNA（lncRNA）人浆细胞瘤转化迁移基因1（PVT1）和MINCR（MYC-induced long non-coding RNA）对淋巴瘤细胞株Raji增殖的影响及其可能机制。方法 合成靶向PVT1、MINCR的小干扰RNA（siRNA）和无关对照siRNA（SC-siRNA）序列。实验分为PVT1-siRNA组、MINCR-siRNA组、PVT1-siRNA+MINCR-siRNA组、无关对照组和细胞组（未转染的Raji细胞）。将各条siRNA转入Raji细胞后，用实时定量RT-PCR方法检测MINCR RNA表达水平；分别用CCK8法、流式细胞仪、Western blot检测细胞增殖、细胞周期和c-Myc蛋白的表达情况。结果 转染MINCR-siRNA后MINCR表达下调，与无关对照组和细胞组相比差异有统计学差异（P<0.05）。PVT1-siRNA联合MINCR-siRNA转染到Raji细胞后，细胞增殖明显受到抑制（P<0.05），细胞周期阻滞于G1期（P<0.05），c-Myc蛋白表达下降（P<0.05），且与单用PVT1-siRNA组、MINCR-siRNA组相比差异均具有统计学意义（P<0.05）。结论 同时下调PVT1和MINCR可以增强对Raji细胞增殖的抑制作用。 |
| 英文摘要: |
| Objective To study the influence of down-regulating long non-coding RNA(lncRNA)plasmacytoma variant translocation 1(PVT1) and MYC-induced long noncoding RNA(MINCR)on the proliferation of lymphoma cells Raji and the probable mechanism. Methods Small interfering RNA(siRNA)targeting against PVT1 and MINCR as well as scramble control siRNA(SC-siRNA)were designed and synthesized chemically.The experiment was divided into PVT1-siRNA group, MINCR-siRNA group, PVT1-siRNA+MINCR-siRNA group, scramble control group and untransfected cells group.Using HiperFect transfection reagent,all of the siRNAs were transfected into Raji cells.The MINCR RNA expression level was detected by real-time RT-PCR.The cell proliferation, the cell cycle and the expression of c-Myc protein were detected by CCK8 assay, Flow cytometry and Western blot, respectively. Results The expression level of MINCR RNA ofMINCR-siRNA group was significantly reduced compared with that of the scramble control group and untransfected cells group(P<0.05).The cell proliferation was significantly inhibited after PVT1-siRNA combining with MINCR-siRNA transfected into Raji cells (P<0.05),which was more obvious than that in PVT1-siRNA group and MINCR-siRNA group(P<0.05).Consistently,the cells were arrested in G1 phase after PVT1-siRNA combining with MINCR-siRNA transfected into Raji cells (P<0.05),and it was more obvious compared with PVT1-siRNA group and MINCR-siRNA group(P<0.05).The expression of c-Myc protein was significantly decreased after PVT1-siRNA combining with MINCR-siRNA transfected into Raji cells(P<0.05),which was more obvious compared with PVT1-siRNA group and MINCR-siRNA group(P<0.05). Conclusion Down-regulating PVT1 and MINCR can enhance the inhibition of proliferation of Raji cells. |
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