| Objective To research activity screening and composition analysis of panaxnotoginseng extracts against glutamate-induced PC12 cells. Methods Glutamate-induced toxicity to PC12 cells was used to model cerebral ischemia injury.The experimental animals were divided into control group, model group, and 6.25,12.50,25,50,100,200 ug/mL drug groups.In addition,the activities of petroleum ether extraction and supercritical CO2 extraction on PC12 cells were detectedby MTT,and the extracts determined andanalysed by GC-MS. Results Compared with control group,the cell growth inhibitory rate increased to nearly 50% for 25 mmol/L glutamate in 24 h.Hence, 25 mmol/L glutamate and 24 h incubation time for glutamate model conditions were chosen in our assays. The difference in cell survival rate between petroleum ether extract group of different concentrations and model group was statistically significant(P<0.05).There was significant difference in cell survival rate between 25,50,100 ug/mL petroleum ether extract group(P<0.05).The difference in cell survival rate between supercritical CO2 extract group of different concentrations and model group was statistically significant(P<0.05).There was significant difference in cell survival rate between 25,50,100 ug/mL supercritical CO2 extract group(P<0.05).There was significant difference in cell survival rate between 50,100 ug/mL supercritical CO2 extract group and petroleum ether extract group(P<0.05).In addition,the content of the panaxynol of supercritical CO2 extract (13.09%) was remarkably higher than that of petroleum ether extract (4.10%) by GC-MS analysis. Conclusion Both petroleum ether extract and supercritical CO2 extract have protective effects on PC12 cells. However,the protective action of supercritical CO2 extract is significantly stronger than that of petroleum ether extract. |
