| Objective To compare the bactericidal efficacy and skinirritationof three kinds of clinical disinfectants. Methods According to technical specification for disinfection, neutralization agent identification experiment, suspension quantitative germicidal test and animal toxicity test were used to observe the germicidal efficacy and irritation of ethanol, iodophorand triclosan on Escherichia coli, Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa. Results The error rate of colony numbers of neutralizer identification experiments between groups 3, 4, 5 was respectively 6.40%, 4.00%, 5.23% and 12.90%, 8.61%, 8.31%, all conformed to the requirements. The selected neutralizer and its concentration were suitable for the germicidal test of samples. Triclosan had the best killing effect on Staphylococcus aureus and Escherichia coli after acting at 0.5 min and 1 min, the average killing log valuewas respectively(6.65±0.05),(7.56±0.03) and(6.62±0.09),(7.51±0.08), and thegermicidal efficacy of three kinds of disinfectants had significant difference at the same time(P<0.05); ethanol had the best killing effect on Pseudomonas aeruginosa and Candida albicans after acting at 0.5 min, 1 min and 1.5 min; the average killing log value was respectively(6.65±0.06),(7.02±0.04),(8.02±0.02) and(6.36±0.04),(7.62±0.10),(8.17±0.02). The germicidal efficacy of three kinds of disinfectants had significant difference at the same time(P<0.05).The irritation index of skin was measured to be 0 and the irritation stimulus intensity was non-irritating of these three kinds of clinical disinfectants. Conclusion The three kinds of clinical disinfectants are safe and non-irritating to skin of guinea pigs. Triclosan hasthe best killing efficacy on Escherichia coli and Staphylococcus aureus. Ethanol hasthe best killing efficacy on Candida albicans and Pseudomonas aeruginosa. |
