| Objective To investigate the effect of chloroquine on tumor growth in mice with hepatic carcinoma and the underlying mechanism. Methods The tumor model of liver cancer mice was established, and 75 mice were randomly divided into control group, low-dose chloroquine treatment group, and the high-dose chloroquine treatment group, then the two chloroquine treatment groups were injected with different dose of chloroquine (25 μmol/L and 50 μmol/L), respectively, and the control group was treated with same amount of saline. After 16 days, the expression of LC3 and P62 in three groups were detected by Western blot and immunohistochemistry, and the proliferation activity of HepG2 treatment with different doses of chloroquine was also analyzed through MTT. Results Compared with control group, the expression of LC3(3.15±0.29) and P62 (6.24±0.41) in chloroquine treatment groups significantly increased, with higher positive cell rate and stronger staining intensity, and the expression of LC3 and P62 also increased with the concentration of chloroquine through Western blot(P<0.05); besides, the proliferation activity of HepG2 treatment with chloroquine was significantly inhibited(P<0.05),showing a significant time-and dose-dependent(P<0.05). Conclusion Chloroquine may promote the apoptosis of tumor or inhibit the proliferation of tumor by inhibiting the autophagic activity of tumor cells. |
