| Objective To investigate the effect of paeoniflorin on the postoperative intestinal adhesion in rat model. Methods Forty male SD rats were selected and randomly divided into four groups:sham, control, hyaluronate (HA) group and paeoniflorin group, with ten in each group. In sham group, the caecum of rats was pulled out and put back after normal laparotomy, and.intestinal adhesion model was established in other groups using cecum friction and the corresponding abdominal wall injury model. Paeoniflorin group received intragastric administration of paeoniflorin and HA group was placed sodium hyaluronate intraperitoneally, then all mice were sacrificed 10 days after the successful model establishment. The degree and scope of adhesion were evaluated and compared among four groups of rats, then the adhesion score was evaluated, and the inflammatory exudation was analyzed pathologically. The expressions of tumor growth factor β (TGF-β) and interleukin-8 (IL-8) were detected by Western blot, and the expression levels of TGF-β and IL-8 were detected by ELISA kit. Results Compared with control group, the degree and scope of adhesion were evaluated by naked eyes 10 days after operation, and the grade and score of adhesion in paeoniflorin group were significantly lower than those in control (P<0.50). HE pathological analysis showed that the inflammatory exudation in paeoniflorin group was significantly lower than that in control(P<0. 05). The TGF-β and IL-8 in paeoniflorin group were significantly lower than those in control (P<0. 05). The expressions of TGF-β and IL-8 in paeoniflorin group were significantly lower than those in paeoniflorin group by Western blotting. Conclusion Paeoniflorin can significantly reduce the TGF-β and IL-8, which is more effective than hyaluronate in anti-intestinal adhesion, and it is expected to be the target drug for prevention of intestinal adhesion after operation. |
