| Objective To investigate the expression of miR-125a-5p in serum of patients with non-small cell lung cancer(NSCLC), and to analyze the correlation between miR-125a-5p and clinicopathological characteristics and its significance in early diagnosisof NSCLC. Methods 44 NSCLC patients in Chaohu Hospital of Anhui Medical University were selected as experimental group and 20 healthy volunteers as control group from July 2017 to March 2018. The expression levels of miR-125a-5p in peripheral blood of the subjects were detected by Q-PCR, and its clinical significance was evaluated. Results The relative expression level of miR-125a-5p in serum of experimental group was 0.025 (0.017,0.051), which was lower than that in control group 0.050(0.034, 0.117), and the difference was statistically significant (P<0.05).The expression of miR-125a-5p in experimental group was negatively correlated with clinical TNM stage (r=-0.688,P<0.05), but not correlated with CEA and cyfra21-1 levels (P>0.05). There was no statistically significant difference in the expression of miR-125a-5p in sex, age, smoking and tissue typing in experimental group. Conclusion MiR-125a-5p is down-regulated in serum of NSCLC patients, which is better than CEA and cyfra21-1 in early diagnosis of NSCLC, and may be an important biological marker for early diagnosis of NSCLC. |
