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| 白细胞介素1β对结直肠癌细胞HCT116迁移能力和上皮-间质转化的影响 |
| Effect ofIL-1β on human colorectal cancer cell HCT116 metastasis and EMT transformation |
| 投稿时间：2019-04-26 |
| DOI：10.3969/j.issn.1000-0399.2019.10.001 |
| 中文关键词: 结直肠癌 白细胞介素1β 上皮间质转化 锌指绑定增强蛋白1 侵袭转移 |
| 英文关键词: Colorectal cancer IL-1β EMT ZEB1 Invasion and metastasis |
| 基金项目:安徽省自然科学基金项目（项目编号：1608085QH217） |
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| 中文摘要: |
| 目的 研究白细胞介素1β（IL-1β）诱导结直肠癌细胞HCT116转移的分子机制。方法 HCT116细胞分为空白对照组（不进行任何处理），IL-1β处理组（加入200 pmol/L IL-1β处理），干扰阴性对照+IL-1β处理组（转染阴性干扰对照再加入IL-1β处理），干扰锌指绑定增强蛋白1（ZEB1）+IL-1β处理组（转染ZEB1干扰RNA）。使用Transwell迁移实验研究各组细胞的迁移能力。荧光定量聚合酶监链式反应（PCR）检测各组细胞中E-cadherin和ZEB1基因的表达。免疫印迹实验检测各组细胞E-cadherin和ZEB1蛋白的表达。结果 空白对照组穿过Transwell小室的细胞数量为（58.3±7.2）个，IL-1β处理组为（129.6±12.1）个，差异有统计学意义（P<0.05）；干扰阴性对照+IL-1β处理组穿过Transwell小室的细胞数量为（108.7±15.9）个，干扰ZEB1+IL-1β处理组为（68.2±11.4）个，差异有统计学意义（P<0.05）。空白对照组HCT116细胞中E-cadherin基因的相对表达量、E-cadherin蛋白相对灰度值均高于IL-1β处理组，差异均有统计学意义（P<0.05）；干扰阴性对照+IL-1β处理组HCT116细胞中E-cadherin基因的相对表达量、E-cadherin蛋白相对灰度值均低于干扰ZEB1+IL-1β处理组，差异均有统计学意义（P<0.05）。结论 IL-1β通过诱导ZEB1表达促进结直肠癌细胞HCT116发生EMT转化并提高其迁移能力。 |
| 英文摘要: |
| Objective To investigate the molecular mechanism of interleukin 1β (IL-1β)-mediated metastasis of colorectal cancer cells. Methods HCT116 cells were divided into control group (without treatment), IL-1β group (IL-1β treatment), si-negative+IL-1β group (transfection with si-negative RNAi+ IL-1β treatment) and si-ZEB1+IL-1β group (transfection with si-ZEB1 RNAi+ IL-1β treatment). Transwell assay was employed to detect the metastasis capability of each group cells. qRT-PCR was used to investigate the mRNA expression of E-cadherin and ZEB1 in each group cells. Western blot was applied to detect the protein expression of E-cadherin and ZEB1 in each group cells. Results The number of Transwell in control group was smaller than that in IL-1β group[(58.3±7.2) vs (129.6±12.1)], and the difference was statistically significant (P<0.05). The number of Transwell of si-ZEB1+IL-1β group was smaller than that in si-negative+IL-1β group[(68.2±11.4) vs (108.7±15.9)], and the difference was statistically significant (P<0.05). The mRNA expression of E-cadherin in IL-1β group was lower than that in control group, and the mRNA expression of E-cadherin in si-ZEB1+IL-1β group was higher than that in si-negative+IL-1β group, all with statistical significance (P<0.05). The protein expression of E-cadherin in IL-1β group was lower than that in control group, and protein expression of E-cadherin in si-ZEB1+IL-1β group was higher than that in si-negative+IL-1β group, all with statistical significance (P<0.05). Conclusions IL-1β induces EMT transformation and metastasis of human colorectal cancer cell HCT116 by up-regulation of ZEB1. |
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