Objective To investigate the expression levels of heat shock protein 90a (Hsp90a) in plasma of patients,carcinoembryonic antigen (CEA),and carbohydrate antigen 199(CA199) in serum of patients with gastric cancer and evaluate their diagnostic and prognostic values.Methods Eighty-six patients with newly diagnosed gastric cancer admitted to the First Affiliated Hospital of Anhui Medical University from January 2018 to January 2019 were chosen as the gastric cancer group.Another 94 healthy subjects from the same physical examination center were randomly extracted as the control group,using enzyme-linked immuneosorbent assay and electrochemistry.The expression levels of Hsp90a,CEA and CA199 in peripheral blood of gastric cancer group and control group were identified by luminescence method.The positive rate,sensitivity and specificity of single and combined detection were analyzed,and subgroup analysis was carried out according to clinical pathological features.Results The expression levels of Hsp90a,CEA and CA199 in gastric cancer group were higher than those in control group[(100.38±74.68) vs (60.70±15.62), (24.11±96.95) vs (2.24±1.51),and (167.96±1 085.37) vs (11.16±15.23)],the positive rate of expressions was also higher than that of control group, and the difference was statistically significant(P<0.05).The sensitivity of single detection of gastric cancer in Hsp90a,CEA and CA199 was 51.16%,30.23% and 16.28%,respectively,and the difference was statistically significant(P<0.05),but there was no significant difference between the specificity groups(P>0.05).The combination of the three tests reached the highest sensitivity and the overall specificity decreased.There was a major positive correlation between the expression levels of CEA and CA199 and the number of lymph node metastasis and clinical stage(P<0.05).There was not any significant correlation between Hsp90a and clinical features such as presence or absence of thoracic and ascites,tumor location,differentiation degree and depth of invasion(P>0.05).Conclusions Hsp90a,CEA,and CA199 are highly expressed in peripheral blood of patients with gastric cancer.The combination detection of the three can get certain diagnostic efficiency in gastric cancer diagnosis. |