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| 成球培养法富集胰腺癌肿瘤干细胞的研究 |
| Enrichment of pancreatic cancer stem cells by sphere-forming culture |
| 投稿时间：2019-10-08 |
| DOI：10.3969/j.issn.1000-0399.2019.12.001 |
| 中文关键词: 细胞成球培养 肿瘤干细胞富集 肿瘤干细胞 胰腺癌 |
| 英文关键词: Sphere-forming culture system Cancer stem cell enrichment Cancer stem cell Pancreatic cancer |
| 基金项目:国家自然科学基金项目（项目编号：81141090） |
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| 中文摘要: |
| 目的 研究成球培养法培养胰腺癌肿瘤干细胞的可行性。方法 通过细胞成球培养体系富集胰腺癌肿瘤干细胞并进行培养，流式细胞术检测分析干细胞的表型特征。结果 光镜下，成球培养细胞形态发生明显变化，细胞由贴壁时的多边形变成圆形，并形成肿瘤细胞微球。流式细胞仪检测发现，与普通细胞培养相比，成球培养体系富集的胰腺癌肿瘤干细胞干性相关基因CD24、CD44共表达水平明显升高。结论 利用成球培养法能够富集得到CD24、CD44高表达的胰腺癌肿瘤干细胞。 |
| 英文摘要: |
| Objective To study the feasibility of sphere-forming culture system for enriching tumor stem cells in normal pancreatic cancer cell line.Methods Pancreatic cancer stem cells were enriched by sphere-forming system. Flow cytometry was applied to determine the stem cell markers expression of the enriched cells. Results In the sphere-forming culture system,the morphology of the cancer stem cells were apparently different from that of normal culture cells under the light microscope.The cells turned small rounded-shaped and formed tumor spheres.The results of flow cytometry showed the co-expression of stemness-related protein CD24 and CD44 was significantly higher in MiaPaca2-CSC than those MiaPaca2 in normal culture.Conclusion Pancreatic cancer stem cells with high expression of CD24 and CD44 can be enriched by sphere-forming culture system. |
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