文章摘要
表没食子儿茶素没食子酸脂联合阿米福汀对小鼠卵巢的辐射保护作用
Radioprotective effect of epigallocatechin-3-gallate combined with amifostine on mice ovary
投稿时间：2020-01-05  
DOI：10.3969/j.issn.1000-0399.2020.08.001
中文关键词: 电离辐射  阿米福汀  表没食子儿茶素没食子酸脂  卵巢
英文关键词: Ionizing  Radiation  Amifostine  Epigallocatechin-3-gallate  Ovary
基金项目:安徽省高校自然科学基金一般项目（项目编号：12925KJ2017B05）
作者单位
李孝鹏 230041 合肥 安徽省第二人民医院放疗科 
王章桂 230041 合肥 安徽省第二人民医院放疗科 
魏南 230041 合肥 安徽省第二人民医院放疗科 
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中文摘要:
      目的 探讨表没食子儿茶素没食子酸脂（EGCG）联合阿米福汀（WR-2721）对小鼠卵巢的辐射保护作用。方法 150只ICR雌性小鼠采用随机数字表法分成Sham组、Control组、WR组、EGCG组与WR+EGCG组，每组30只。除Sham组外，其余小鼠均接受6 Gy X线照射，在治疗后第8小时、7天和14天，分别测定每组小鼠的体质量、血细胞计数、卵巢指数及细胞凋亡，观察卵巢形态变化。结果 Control组小鼠辐射后体质量下降，Sham组小鼠体质量持续增长，WR、EGCG、WR+EGCG组小鼠体质量缓慢上升，差异有统计学意义（P<0.05）。Control组小鼠辐射后白细胞、红细胞、血小板水平较其余各组均下降，差异有统计学意义（P<0.05），各组小鼠血红蛋白水平辐射后变化不大（P>0.05）。Control组卵泡数量明显减少，出现大量闭锁卵泡或空卵泡，WR、EGCG组存在少量的闭锁卵泡或空卵泡，WR+EGCG组未见闭锁卵泡或空卵泡。Control组小鼠卵巢指数显著下降，EGCG组及WR+EGCG组小鼠卵巢指数高于Control组，差异有统计学意义（P<0.05）。Control组TUNEL染色阳性的闭锁卵泡及凋亡颗粒细胞最多。与Sham组比较，接受辐射的卵巢颗粒细胞凋亡指数均升高，Control组最高，EGCG组和WR组凋亡指数升高介于Control组及WR+EGCG组之间，差异有统计学意义（P<0.05）。结论 EGCG联合阿米福汀可以增强小鼠卵巢的辐射保护作用。
英文摘要:
      Objective To investigate the protective effect of epigallocatechin-3-gallate(EGCG) and amifostine(WR-2721) on mouse ovaries against ionizing radiation. Methods One hundred and fifty female ICR mice were randomly divided intosham group, control group, WR group,EGCG group, and WR + EGCG group. Each group consisted of thirty animals.Except for sham group, the mice in the other groups were irradiated by X-rays with a dose of 6 Gy. At 8 hours, 7 days and 14 days after treatment respectively, the body weight, blood cell count, ovarian index and apoptosis of mice in each group were measured, and the changes of ovarian morphology were observed. Results After radiation, the body weight of mice in control group decreased, while that in sham group continued to increase and in WR, EGCG and WR + EGCG groups increased slowly,and the difference was statistically significant (P<0.05).The levels of white blood cells, red blood cells and platelets in control group were lower than those in the other groups (P<0.05), but the hemoglobin level of the mice in control group did not change significantly (P>0.05).The number of follicles in control group decreased significantly, and a large number of atresia follicles or empty follicles appeared. There were a small number of atresia or empty follicles in WR and EGCG groups, but no atresia or empty follicles were found in WR + EGCG group. The ovarian index of control group was significantly lower than that of control group, and the ovarian index of EGCG group and WR + EGCG group was higher than that of control group (P<0.05).There were moreatretic follicles and apoptotic granulosa cells stained positive for TUNEL labelling in control group.Compared with sham group, the apoptosis index of ovarian granulosa cells in radiation group was higher than that in control group, and the apoptosis index of EGCG group and WR group was higher than that of control group and WR + EGCG group (P<0.05). Conclusion EGCG combined with WR-2721 enhances the protection of ovaries against ionizing radiation in mice.
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