| Objective To investigate the expressions of miR-219 and calcium/calmodulin dependent protein kinase II-γ (CaMK II-γ) in peripheral blood of patients with Alzheimer's disease (AD) and their significance. Methods Seventy-nine AD patients were selected from February 2016 to February 2019 in our hospital. According to the clinical dementia scale, they were were divided into 29 cases in mild group, 24 cases in moderate group and 26 cases in severe group. During the same period, 79 healthy persons in our hospital were selected as control group. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the levels of miR-219 and CaMK II-γ in peripheral blood, enzyme-linked immunosorbent assay (ELISA)was employed to detect the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and Pearson analysis was applied to analyze the correlations among the levels of miR-219, CaMK II-γ, IL-6 and TNF-α. Results Compared with control group, the levels of serum CaMK II-γ, IL-6 and TNF-α in AD group were higher (P<0.05)and the level of serum miR-219 was lower (P<0.05); compared with mild group, the levels of serum CaMK II-γ mRNA, IL-6 and TNF-α were higher in moderate group and severe group (P< 0.05), and the level of miR-219 was lower (P<0.05); compared with moderate group, the levels of serum CaMK II-γ mRNA, IL-6 and TNF-α were higher in severe group (P<0.05) and the level of miR-219 was lower (P<0.05). All the differences were statistically significant. Pearson analysis showed that miR-219 was negatively correlated with CaMK II-γ, IL-6 and TNF-α (r=-0.546, -0.584, P<0.05), while CaMK II-γ was positively correlated with IL-6 and TNF-α (r=0.523, 0.603, P<0.05). Conclusion Serum CaMK II-γ mRNA level is up-regulated in patients with Alzheimer's disease, and the level of miR-219 is down-regulated, which is related to the severity of the disease and may be related to the occurrence and progress of Alzheimer's disease. |
