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| RapidWarm Blast玻璃化解冻液对囊胚复苏的效果观察 |
| Effects of RapidWarm Blastvitrification mediums on recovery of blastocysts in frozen-thaw cycles |
| 投稿时间：2020-03-23 |
| DOI：10.3969/j.issn.1000-0399.2020.11.011 |
| 中文关键词: 胚胎解冻周期 玻璃化 解冻液 囊胚 胚胎复苏 |
| 英文关键词: Frozen-thaw cycle Vitrification Thawing medium Blastocyst Embryo recovery |
| 基金项目:国家重大科研仪器研制项目(项目编号:11627803),国家自然科学基金项目(项目编号:82001516) |
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| 中文摘要: |
| 目的 观察在囊胚复苏周期中,RapidWarm Blast玻璃化解冻液对囊胚的复苏效果。方法 回顾性分析2018年12月至2019年12月在安徽医科大学第一附属医院首次行囊胚复苏移植的655例患者临床资料,根据囊胚解冻液不同分A组357例及B组298例。A组采用传统Vitrification Media解冻液进行囊胚复苏,B组采用新型RapidWarm Blast解冻液复苏。比较两组囊胚复苏后整体存活率,解冻2 h后囊胚重扩张率、孵化率,囊胚种植率及临床妊娠率;同时比较两种解冻液对囊胚不同发育阶段(第5天囊胚、第6天囊胚)的重扩张率、孵化率及临床妊娠率的影响。结果 两组囊胚复苏后的存活率、重扩张率、孵化率、临床妊娠率比较,差异均无统计学意义(P>0.05);B组囊胚种植率(55.45%)高于A组(48.76%),差异有统计学意义(P<0.05)。两组中发育第5天的囊胚复苏2 h后重扩张率及孵化率均高于发育第6天的囊胚,差异均有统计学意义(P<0.05);两组间发育第5天的囊胚复苏2 h后重扩张率及孵化率比较,差异无统计学意义(P>0.05),B组发育第6天的囊胚复苏2 h后扩张率及孵化率均高于A组,差异有统计学意义(P<0.05)。结论 新型RapidWarm Blast解冻液用于囊胚玻璃化复苏,可获得与传统的Vitrification Media相似的临床结局,但新型解冻液对发育第6天囊胚的复苏效果更好,胚胎种植率更高。 |
| 英文摘要: |
| Objective To observe the clinical effects of blastocyst recovery in frozen-thaw cycles using thawing medium (RapidWarm Blast). Methods From December 2018 to December 2019, a total of 655 patients who underwent their first frozen-thaw cycles in the Reproductive Medicine Center were recruited for retrospective analysis, of which, 357 patients with the blastocysts recovery using Vitrification Media were set as Group A, while 298 patients with the blastocysts recovery using with RapidWarm Blast were set as Group B. The overall survival rate, re-expansion rate, hatching rate, implanting rate and clinical pregnancy rate of blastocysts after thawing were compared between the two groups. Meanwhile, the recovery effects and clinical pregnancy outcomes of blastocysts with different development days D5/D6 were investigated. Results The differences in survival rates, re-expansion rates, hatching rates, and clinical pregnancy rates between Group A and Group B after blastocyst recovery were not statistically significant (P>0.05). The implantation rate of Group B (55.45%) was significantly higher than that ofGroup A (48.76%). The re-expansion and hatching rates of D5 blastocysts were significantly higher than those ofD6 in both groups(P<0.05). The recovery effects on D5 blastocysts were not different between two groups (P>0.05), while D6 blastocyst recovery in Group B was higher than that in Group A(P<0.05). Conclusions The effects of blastocyst recovery arestable, consistent and equal between the two different vitrification/thawing mediums. RapidWarm Blasthas better recovery effects and higher embryo implantation rate on D6 blastocysts. |
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