Objective Tostudy the correlation of intestinal flora, short-chain fatty acids (SCFAs) and blood lipids in Tibetan patients with coronary heart disease for the individualized clinical treatment.Methods From August 2017 to February 2018, 30 Tibetan with suspected CHD were hospitalized in the Department of Coronary Heart Disease in Qinghai Cardio-cerebrovascular Disease Special Hospital and enrolled in this study. According to the results of CT and coronary angiography, the inpatients with single or multiple coronary artery stenosis ≥ 50% were divided into CHD group (CHD group, 18 patients), and the remaining patients were included in patient control group (NCHD group, 12 patients). Another 23 Tibetan volunteers served as the healthy control group (HT group). Fasting serum and fecal samples were collected from the subjects in the three groups. The faecal microbiota of all samples were profiled by 16S rDNA. The content of SCFAs was detected by a full-automatic gas chromatography-mass spectrometer, and the serum lipid level was detected by a full-automatic blood biochemical analyzer.The relationship between bacteria, blood lipids and short-chain fatty acidswas analysed by Spearman correlation analysis. Results High density lipoprotein cholesterol (HDL-c) in CHD group and NCHD group was lower than that in HT group, and the level of fecal isobutyric acid in NCHD group was higher than that in HT group, the differences were statistically significant(P<0.05). Compared with HT group, the relative abundance of Dialister increased in CHD group and NCHD group, while the relative abundance of Blautia decreased. Compared with NCHD group, the relative abundance of Bacteroides in CHD group decreased, and the differences were statistically significant (P<0.05). Spearman correlation analysis showed that HDL-c level was positively correlated with fecal isobutyric acid level in CHD group (r=0.808,P<0.01).There was a negative correlation between isobutyric acid level and relative abundance of Prevotella in HT group (r=-0.438, P<0.05), and a positive correlation with the relative abundance of Blautia (r=0.467, P<0.05). The relative abundance of Blautia in NCHD group was positively correlated with the level of isobutyric acid (r=0.627, P<0.05).Conclusions The composition of intestinal flora, the levels of blood lipids and short-chain fatty acids are changed in Tibetan patients with coronary heart disease, the sobutyric acid level iscorrelated with the abundance of flora and blood lipid levels. |