文章摘要
长链非编码RNA-EPIC1在前列腺癌中的表达及其临床意义
Relationship between long non-coding RNA-EPIC1 with lymph node metastasis and prognosis in prostate cancer
投稿时间：2020-12-17  
DOI：10.3969/j.issn.1000-0399.2021.07.001
中文关键词: 表观遗传诱导LncRNA-1  前列腺癌  细胞增殖  生存率
英文关键词: EPIC1  Prostate cancer  Cell proliferation  Survival rate
基金项目:国家自然科学基金（项目编号：81500576、82004010）
作者单位
唐智国 238000 安徽省合肥市第八人民医院(合肥职业技术学院附属医院)泌尿外科 
魏灿 230011 安徽省合肥市第二人民医院泌尿外科 
邰胜 230022 合肥 安徽医科大学第一附属医院泌尿外科 
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中文摘要:
      目的 探讨表观遗传诱导LncRNA-1（EPIC1）在前列腺癌（PCa）中的表达情况及其临床意义。方法 收集2017年3月至2020年1月在合肥市第八人民医院、合肥市第二人民医院及安徽医科大学第一附属医院确诊的10例PCa患者手术后癌组织和癌旁正常组织，通过实时定量聚合酶链反应（RT-PCR）分别检测EPIC1在患者手术组织、正常前列腺细胞系和PCa细胞系中的表达差异。用siRNA技术抑制PCa细胞系中EPIC1的表达，观察其对细胞增殖和侵袭能力的影响。另检测69例PCa患者癌组织中EPIC1的表达情况，分析EPIC1的表达水平与患者临床病理和生存资料之间的关系。结果 EPIC1在PCa细胞系PC3、DU145中的表达量分别为（0.12±0.02）和（0.20±0.03），高于在正常前列腺细胞系RWPE-1中的表达量，差异有统计学意义（P<0.05）。EPIC1在患者PCa组织中的平均表达量为（0.61±0.05），高于在癌旁正常组织中的平均表达量，差异有统计学意义（P<0.05）。用siRNA抑制EPIC1的表达后，PCa细胞的增殖和侵袭能力下降；EPIC1的表达程度与肿瘤的Gleason评分、TNM分期和淋巴结转移明显相关（χ2=4.949、6.040、5.650，P<0.05），但与患者年龄、PSA水平等无明显相关性（χ2=0.007、1.516，P>0.05）。以EPIC1的平均值为界，将患者分为EPIC1高表达组和低表达组，生存分析结果显示，EPIC1高表达组和低表达组患者的3年生存率分别为53.7%、78.1%，两组生存率的差异有统计学意义（P<0.05）。结论 EPIC1在PCa中表达增高，EPIC1能促进PCa细胞的增殖和侵袭，EPIC1高表达的患者预后较差，EPIC1可做为PCa肿瘤转移和判断预后的有效辅助指标。
英文摘要:
      Objective To investigate the expression and clinical significance of epigenetically induced longnon-coding RNA1(EPIC1) in prostate cancer(PCa). Methods Ten PCa patients were selected in the Eighth People's Hospital of Hefei, the Second People's Hospital of Hefei and the First Affiliated Hospital of Anhui Medical University during March, 2017 to January, 2020. The levels of EPIC1 were detected in PCa tissues, corresponding adjacent normal tissues, normal prostate cell line and two PCa cell lines via real-time polymerase chain reaction(RT-PCR) method. The expression of EPIC1 in PCa cells was inhibited. The effect of EPIC1 on cell proliferation and invasion was observed with siRNA technique. The relationship between EPIC1 and clinical pathology and survival data of 69 PCa patients was analyzed. Results The level of EPIC1 in PCa cell lines PC3 and DU145 was (0.12±0.02) and (0.20±0.03) respectively, while only about (0.01±0.001) in normal prostate cell line RWPE-1. The expression of EPIC1 in PCa cell lines was significantly higher than that inormal prostate cell lines(P<0.05). The average expression of EPIC1 in PCa tissues of patients was (0.61±0.05), while that in normal adjacent tissues was only (0.056±0.003), and the difference was significant(P<0.05).After inhibiting the expression of EPIC1 with siRNA, the proliferation and invasiveness of PCa cells decreased. The expression of EPIC1 was significantly correlated with Gleason score, TNM stage and lymph node metastasis (χ2=4.949, 6.040,5.650, P<0.05),but not clearly with age or PSA level (χ2=0.007, 1.516, P>0.05). The patients were divided into high-EPIC1 group and low-EPIC1 group based on the average level of EPIC1, and survival analysis showed that the 3-year survival rate of patients with high and low expression of EPIC1 was 53.7% and 78.1%, respectively. The difference in the survival rate of the two groups was statistically significant(P<0.05). Conclusions The level of EPIC1 increases in PCa tissues,EPIC1 can promote the proliferation and invasion of PCa cells, and over expression of EPIC1 indicates a poor prognosis in PCa patients. EPIC1 can be used as an effective index for evaluating metastasis and prognosis of PCa.
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