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| 牛奶蛋白过敏患儿维生素D代谢酶和其受体水平 |
| Study ofvitamin D metabolizing enzymes and vitamin D receptorin children with milk protein allergy |
| 投稿时间：2021-09-12 |
| DOI：10.3969/j.issn.1000-0399.2022.05.011 |
| 中文关键词: 牛奶蛋白过敏 维生素D 维生素D代谢酶 维生素D受体 |
| 英文关键词: Milk protein allergy Vitamin D Vitamin D metabolizing enzymes Vitamin D receptor |
| 基金项目:安徽医科大学校科研基金(项目编号:2019xkj185) |
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| 中文摘要: |
| 目的 探讨牛奶蛋白过敏(CMPA)患儿外周血单个核细胞维生素D代谢酶(CYP24A1、CYP27B1)和其受体(VDR)水平。方法 选择2018年5月至2020年5月安徽省儿童医院32例 CMPA患儿为研究对象(试验组),根据症状将患儿分为轻中度组和重度组,将同期进行健康体检的17例健康婴幼儿作为对照组,比较试验组和对照组,轻中度组和重度组25羟维生素D 水平、VDR、CYP24A1、CYP27B1转录水平差异。结果 ① 试验组25(OH)D水平低于对照组,重度组患儿25(OH)D水平低于轻中度组,差异具有统计学意义(P<0.05);② 试验组CYP24A1转录水平高于对照组,重度组患儿CYP24A1转录水平高于轻中度组, 差异具有统计学意义(P<0.05);③CYP27B1、VDR转录水平在试验组和对照组、轻中度组和重度组之间相比,差异无统计学意义(P>0.05);④试验组患儿25(OH)D水平与住院天数呈负相关(r=-0.470, P=0.007); CYP24A1转录水平与住院天数呈正相关(r=0.806, P<0.001),25(OH)D水平与CUP24A1转录水平呈负相关(r=-0.397,P=0.024)。结论 CMPA患儿25(OH)D水平降低与CYP24A1水平升高有关,CYP24A1可能是CMPA潜在的治疗靶点。 |
| 英文摘要: |
| Objective To investigate the levels of vitamin D metabolizing enzymes (CYP24A1, CYP27B1) and receptors (VDR) in peripheral blood mononuclear cells of children with milk protein allergy (CMPA).Methods From May 2018 to May 2020, 32 children diagnosed with CMPA were selected as subjects in Anhui Provincial Children's Hospital (experimental group), and the children were divided into mild-moderate group and severe group according to their symptoms, while 17 cases of healthy children were collected as an control group during the same period. The transcriptional level of 25-hydroxyvitamin D[25(OH)D], VDR, CYP24A1, and CYP27B1 in the experimental group and control group, mild-moderate group and severe group was measured and compared. Results ①The level of 25(OH)D in experimental group was lower than that of control group, the level of 25(OH)D in severe group was lower than that of the mild-moderate group, and the differences were statistically significant (P<0.05).②The transcriptional level of CYP24A1 in the experimental group was higher than that of the control group, and the transcriptional level of CYP24A1 in severe group was higher than that of the mild-moderate group, and differences were statistically significant (P<0.05).③There was no significant difference in CYP27B1 and VDR transcription levels between the experimental group and control group, or between the mild-moderate group and severe group (P>0.05).④ 25(OH)D level in the experimental group was negatively correlated with the time used in hospital (r=-0.470, P=0.007), andthe transcription level of CYP24A1 was positively correlated with the time used in hospital (r=0.806, P<0.001). Conclusions The level of 25(OH)D in CMPA is significantly reduced, which is caused by increased transcription of CYP24A1.CYP24A1 is a potential therapeutic target of CMPA. |
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