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| 碳青霉烯诱导对多重耐药鲍曼不动杆菌青霉素结合蛋白编码基因表达的影响 |
| Effect ofcarbapenem inductionon expression of penicillin-binding protein encoding gene of multidrug-resistant Acinetobacter baumannii |
| 投稿时间：2020-11-18 |
| DOI：10.3969/j.issn.1000-0399.2022.06.009 |
| 中文关键词: 鲍曼不动杆菌 多重耐药 青霉素结合蛋白 碳青霉烯 |
| 英文关键词: Acinetobacter baumannii Multiple drug resistance PBPs Carbapenem |
| 基金项目:四川省卫计委基金资助项目(项目编号:18PJ108),四川省基层卫生事业发展研究中心2020年立项项目(项目编号:SWFZ20-C-070),成都医学院校级课题(项目编号:CYZYB20-23) |
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| 中文摘要: |
| 目的 探讨碳青霉烯诱导对多重耐药鲍曼不动杆菌(MDR-AB)青霉素结合蛋白(PBPs)编码基因表达的影响。方法 收集2018年6月至2018年12月成都市郫都区人民医院临床分离的19株MDR-AB菌株,聚合酶链式反应(PCR)扩增所有菌株7种PBPs 基因。分别用1 mg/L浓度的亚胺培南、美罗培南、多尼培南对菌株进行诱导处理,对照组为未加任何抗生素的空白组。实时定量荧光PCR(qRT-PCR)检测7种PBPs基因的表达情况,采用相对定量(2-△△Ct)法比较碳青霉烯诱导后MDR-AB的7种PBPs基因表达量与对照组的差异。结果 19株MDR-AB均携带7种PBPs基因,其中1 mg/L亚胺培南诱导后MDR-AB中pbp1a,pbp2,pbp3,pbp7基因相对表达量分别为(0.41±0.12)、(0.55±0.25)、(0.55±0.46)、(0.62±0.39),与对照组相比基因表达水平下降,差异有统计学意义(P<0.05),pbp1b,pbp5/6,pbp6基因相对表达量分别为(4.31±0.54)、(5.63±1.42)、(8.98±1.14),基因表达水平上调(P<0.05),1 mg/L美罗培南诱导后MDR-AB中pbp2,pbp3基因表达下调,分别为(0.60±0.21)、(0.54±0.47),与对照组比较差异有统计学意义(P<0.05),pbp1b,pbp5/6,pbp6基因相对表达量分别为(2.78±0.21)、(5.66±0.89)、(11.10±1.02),与对照组相比上调,差异有统计学意义(P<0.05),1 mg/L多尼培南诱导后pbp7基因相对表达量为(0.51±0.42),与对照组相比基因表达下降,差异有统计学意义(P<0.05),pbp1b,pbp5/6,pbp6基因相对表达量分别为(7.18±1.20)、(11.66±1.82)、(10.12±0.87),与对照组相比基因表达上调,差异有统计学意义(P<0.05)。结论 MDR-AB普遍携带PBPs基因,亚最小抑菌浓度(MIC)的碳青霉烯诱导可导致MDR-AB的PBPs编码基因表达明显变化。 |
| 英文摘要: |
| Objective To investigate the effect of carbapeneminduction on the expression of penicillin-binding protein (PBPs) encoding gene of multidrug-resistant Acinetobacter baumannii (MDR-AB). Methods Nineteen MDR-AB strains clinically isolated fromthe People’s Hospital of Chengdu's Pidu District from June 2018 to December 2018 were collected, and seven PBPs genes of all the strains were amplified by PCR. The strains were induced with imipenem, meropenem and donipenem at the concentration of 1 mg/L, respectively. The control group was given no antibiotics.The expression of 7 PBPs genes in MDR-AB was detected by real-time quantitative fluorescence PCR(qRT-PCR). The expression of 7 PBPs genes in MDR-AB before and after carbapenem induction was compared by relative quantitative (2-△△Ct) method. Results Seven PBPs genes were successfully amplified in 19 MDR-AB strains. After 1 mg/L imipenem induction,the relative expression levelof PBP1a, PBP2, PBP3 and PBP7 was (0.41±0.12), (0.55±0.25), (0.55±0.46) and(0.62±0.39)(P<0.05), respectively, and the relative expression level of PBP1b, PBP5/6 and PBP6 was(4.31±0.54), (5.63±1.42) and (8.98±1.14)respectively.Compared to the control group significant up-regulation could be observed (P<0.05).PBP2 and PBP3 gene expression was down-regulated in AB after 1 mg/L meropenem induction [(0.60±0.21), (0.54±0.47), respectively)(P<0.05). PBP1b, PBP5/6 and PBP6 was(2.78±0.21),( 5.66±0.89), (11.10±1.02), respectively, which was significantly up-regulatedcompared to the control group (P<0.05).The relative expression of PBP7 gene was (0.51±0.42) after 1 mg/L donipenem induction, which was significantly decreased (P<0.05). PBP1b, PBP5/6 and PBP6 was(7.18±1.20),(11.66±1.82),(10.12±0.87),respectively,which was significantly up-regulatedcompared to the control group (P<0.05). Conclusion MDR-AB generally carries PBPs gene, and sub-MIC of carbapenem can lead to significant changes in the expression of MDR-AB’s PBPs gene. |
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