| Objective To investigate the expression of miR-24-3p and BHLHE22 in endometrial cancer (EC) and their relationship with postoperative recurrence and metastasis.Methods From June 2018 to Dec 2022, 134 EC patients were prospectivelyselected as the EC group from Baoding Maternal and Child Health Hospital, and 134 patients with benign endometrial hyperplasia were selected as the control group. Real-time fluorescence quantitative polymerase chain reaction (qRT PCR) was applied to determine the expression levels of serum miR- 24-3p and BHLHE22. Pearson method was applied to analyze the relationship between serum miR-24-3p and BHLHE22. COX regression was employed to assess the factors affecting postoperative recurrence and metastasis in EC patients. The predictive value of serum miR-24-3p and BHLHE22 for postoperative recurrence and metastasis in EC patients was analyzed by plotting receiver operating characteristic (ROC) curves.Results Compared with the control group, the serum miR-24-3p expression level in the EC group increased (P＜0.05), while the BHLHE22 expression level decreased (P＜0.05). TargetScanHuman website predicted the presence of binding sites between miR-24-3p and BHLHE22. The expression levels of miR-24-3p and BHLHE22 in the serum of EC patients were negatively correlated (r=-0.491, P＜0.001). Compared with thenon-recurrence and metastasis group, the expression level of miR-24-3p in the serum of EC patients in recurrence and metastasis group increased (P＜0.05), while the expression level of BHLHE22 decreased (P＜0.05). There was anobvious difference in lymph node metastasis and distant metastasis between thenon-recurrenceand metastasis group and the recurrence and metastasis group (P＜0.05). BHLHE22 was a protective factor for postoperative recurrence and metastasis in EC patients (P＜0.05), while miR-24-3p, lymph node metastasis, and distant metastasis were risk factors for postoperative recurrence and metastasis in EC patients (P＜0.05). The combined prediction of serum miR-24-3p and BHLHE22 had the highest area under the curve (AUC) for postoperative recurrence and metastasis in EC patients, which was better than the individual prediction of serum miR-24-3p and BHLHE22 (Zcombination-miR-24-3p=3.434, P＜=0.001; Zcombination-BHLHE22=2.281, P= 0.023).Conclusion The serum miR-24-3p level in EC patients obviously increases, while BHLHE22 level apparently decreases, which is related to postoperative recurrence and metastasis.The combination of the two has a higher predictive value for postoperative recurrence and metastasis in EC patients. |
