| [Abstract] Objective To investigate the expression and clinical significance of CD4+T, CD8+T, Treg and Th17 cells in the peripheral blood of gastric cancer patients during the perioperative period. Methods Thirty-two patients with gastric cancer admitted to East District of the First Affiliated Hospital of Anhui Medical University from January 2022 to December 2023 were selected as the observation group, and 12 healthy volunteers who came to the hospital for physical examination in January 2022 as the control group. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to detect CD4+ T, CD8+ T, Treg and Th17 cells in the observation group and the control group,and then calculate the CD4+/CD8+and Treg/Th17 ratios. The TGF-β and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA) in the preoperative observation group and postoperative observation group of radical gastrectomy, and the FOXP3 and RORγt were measured by real-time polymerase chain reaction (RT-PCR). The correlation between Treg and Th17 cells were analyzed by Pearson correlation analysis. The peripheral blood Treg and Th17 cell counts of patients with different clinical stages were compared in the observation group after surgery.Results Prior to surgery, the observation group had higher levels of CD8+T, Treg, and Th17 cells compared to the control group, while CD4+T, CD4+/CD8+, and Treg/Th17 cells were lower than those in the control group (P<0.01);After radical gastrectomy for gastric cancer in the observation group, CD8+T, Treg, Th17 cells and TGF-β, IL-6, FOXP3, RORγt were lower than before surgery, while CD4+T, CD4+/CD8+, Treg/Th17 were higher than before surgery (P<0.05); There was a linear positive correlation between preoperative Treg and Th17 in the observation group (r=0.519, P<0.01); The Treg and Th17 levels in the observation group of postoperative stage I+II patients were lower than those in stage III patients (P<0.05).Conclusions CD4+T, CD8+T, Treg, and Th17 cells play important immune roles in gastric cancer patients, and surgical treatment can affect their expression levels. |
