|
| 血清miR-98-5p和miR-335-5p表达与癫痫患儿认知功能损害的关系 |
| Relationship between serum mir-98-5p and mir-335-5p expression and cognitive impairment in paediatric epilepsy patients |
| 投稿时间：2025-01-17 |
| DOI：10.3969/j.issn.1000-0399.2026.01.011 |
| 中文关键词: 癫痫 儿童 认知功能 miR-98-5p miR-335-5p |
| 英文关键词: Epilepsy Children Cognitive function miR-98-5p miR-335-5p |
| 基金项目:河北省卫生健康委医学科学研究(编号:20220520) |
|
| 摘要点击次数: 338 |
| 全文下载次数: 110 |
| 中文摘要: |
| 目的 探究微小核糖核酸-98-5p（miR-98-5p）、微小核糖核酸-335-5p（miR-335-5p）表达与癫痫患儿认知功能损害的相关性。方法 选择2022年9月至2023年12月在邯郸市中心医院儿科就诊的120例癫痫患儿为研究对象（癫痫组），根据韦氏儿童智力量表评分，将认知功能损害患儿60例作为认知功能损害组，无认知功能损害患儿60例作为无认知功能损害组，同时纳入在本院体检的健康儿童60例。采用定量反转录聚合酶链反应（RT-qPCR）检测各组miR-98-5p、miR-335-5p表达，受试者工作特征（ROC）曲线分析miR-98-5p、miR-335-5p对癫痫患儿认知功能损害的预测价值，Spearman法分析认知功能损害患儿血清miR-98-5p、miR-335-5p水平与韦氏儿童智力量表评分的相关性。多因素logistic回归分析癫痫患儿发生认知功能损害的影响因素。结果 3组儿童miR-98-5p、miR-335-5p表达比较，差异有统计学意义（P<0.05）。与对照组相比，癫痫患儿血清miR-98-5p、miR-335-5p表达水平降低（P<0.05）。不同发病频率、发作形式、病程和脑电图情况的癫痫患儿miR-98-5p、miR-335-5p表达比较，差异均有统计学意义（P<0.05）。血清miR-98-5p、miR-335-5p单独及二者联合诊断癫痫患儿认知功能损害的曲线下面积分别为0.751（95%CI:0.664～0.826）、0.864（95%CI:0.762～0.901）和0.919（95%CI:0.855～0.961）；认知功能损害组患儿韦氏儿童智力量表评分与miR-98-5p、miR-335-5p呈正相关(rmiR-98-5p=0.496,rmiR-335-5p=0.515,P<0.001)。miR-98-5p（OR=0.621）、miR-335-5p（OR=0.518）表达水平均是癫痫患儿发生认知功能损害的重要影响因素（P<0.05）。结论 癫痫认知功能损害患儿血清中miR-98-5p、miR-335-5p表达水平下调，二者联合检测对癫痫患儿合并认知功能损害的诊断效能更高。 |
| 英文摘要: |
| Objective To investigate the correlation between the expression of microribonuclease-98-5p（miR-98-5p） and microribonuclease-335-5p（miR-335-5p） with cognitive impairment in children with epilepsy. Methods A cohort of 120 paediatric epilepsy patients attending the Department of Paediatrics at Handan Central Hospital between September 2022 and December 2023 formed the study population（Epilepsy Group）. Based on Wechsler Intelligence Scale for Children scores, 60 children with cognitive impairment constituted the cognitive impairment group, while 60 children without cognitive impairment formed the non-cognitive impairment group. Additionally, 60 healthy children undergoing physical examinations at the hospital were included. Reverse transcription quantitative polymerase chain reaction（RT-qPCR） was employed to detect miR-98-5p and miR-335-5p expression. Receiver operating characteristic（ROC） curve was employed to assess the predictive value of miR-98-5p and miR-335-5p in predicting cognitive impairment in paediatric epilepsy patients. Spearman's correlation analysis was used to assess the relationship between serum miR-98-5p and miR-335-5p levels and Wechsler Intelligence Scale for Children scores. Multivariate logistic regression was employed to identify the factors influencing cognitive impairment in paediatric epilepsy patients. Results A statistically obvious difference was detected in the expression of miR-98-5p and miR-335-5p among the three groups（P<0.05）. Compared with the control group, the expression levels of serum miR-98-5p and miR-335-5p in children with epilepsy were reduced（P<0.05）. The expressions of miR-98-5p and miR-335-5p had statistically significant difference among epileptic children with different incidence frequencies, seizure forms, disease duration, and EEG conditions（P<0.05）. The area under the curve（AUC） of serum miR-98-5p, miR-335-5p alone and in combination for diagnosing cognitive impairment in children with epilepsy was 0.751（95% CI: 0.664～0.826）, 0.864（95% CI: 0.762～0.901）, and 0.919（95% CI: 0.855～0.961）, respectively. The Wechsler Intelligence Scale for Children score of children with epilepsy combined with cognitive impairment was positively correlated with miR-98-5p and miR-335-5p(rmiR-98-5p=0.496, rmiR-335-5p=0.515, P<0.05). The expression levels of miR-98-5p（OR=0.621） and miR-335-5p（OR=0.518） were both significant factors influencing cognitive impairment in paediatric epilepsy patients（P<0.05）. Conclusion The expression levels of miR-98-5p and miR-335-5p in the serum of children with cognitive impairment in epilepsy are obviously downregulated. The combined detection of the two has a higher diagnostic efficacy for children with epilepsy complicated with cognitive impairment. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
