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| 血清lncRNA MALAT-1 miR-145-5p水平与UC患者疾病活动度肠道菌群分布及复发的关系 |
| Relationship between serum lncRNA MALAT-1, miR-145-5p and disease activity, gut microbiota distribution, and recurrence in patients with ulcerative colitis |
| 投稿时间：2025-04-08 |
| DOI：10.3969/j.issn.1000-0399.2026.01.016 |
| 中文关键词: 溃疡性结肠炎 肺腺癌转移相关转录本1 微小RNA-145-5p 疾病活动度 肠道菌群分布 复发 |
| 英文关键词: Ulcerative colitis Metastasis-associated lung adenocarcinoma transcript 1 MicroRNA-145-5p Disease activity Gut microbiota distribution Recurrence |
| 基金项目: |
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| 摘要点击次数: 302 |
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| 中文摘要: |
| 目的 探讨血清长链非编码RNA肺腺癌转移相关转录本1（lncRNA MALAT-1）、miR-145-5p水平与溃疡性结肠炎（UC）患者疾病活动度、肠道菌群分布及复发的关系。方法 选取2021年1月至2023年12月期间在邯郸市中医院脾胃二科就诊的122例UC患者为UC组，根据复发情况分为非复发组（92例）和复发组（30例）；同期选取100名体检健康者为对照组。根据Mayo评分评估疾病活动度；采用16srRNA测序技术检测两组肠道菌群，并比较两组肠道菌群门、属水平相对丰度；实时荧光定量PCR法检测两组血清lncRNA MALAT-1、miR-145-5p水平；Pearson法分析UC患者肠道菌属与血清lncRNA MALAT-1、miR-145-5p的相关性，ENCORI网站预测二者之间是否存在靶向关系；多因素logistic回归分析UC患者预后影响因素；受试者工作特征（ROC）曲线分析血清lncRNA MALAT-1和miR-145-5p水平对UC患者复发的预测价值。结果 与对照组相比，UC组血清lncRNA MALAT-1水平明显升高（P<0.05）,miR-145-5p水平明显降低（P<0.05）。lncRNA MALAT-1和miR-145-5p存在靶向结合位点，且UC患者血清中lncRNA MALAT-1和miR-145-5p表达呈负相关（r=-0.536,P<0.05）。与轻度活动期、缓解期患者相比，中重度活动期患者血清lncRNA MALAT-1水平升高（P<0.05）,miR-145-5p水平降低（P<0.05）。门水平上，UC患者厚壁菌门相对丰度明显降低（P<0.05），变形菌门相对丰度明显升高（P<0.05）；属水平上，乳杆菌属、双歧杆菌属相对丰度明显降低（P<0.05），肠球菌属、大肠杆菌属相对丰度明显升高（P<0.05）。乳杆菌属、双歧杆菌属与血清lncRNA MALAT-1水平呈负相关（P<0.05），与miR-145-5p水平呈正相关（P<0.05）；肠球菌属、大肠杆菌属与血清lncRNA MALAT-1水平呈正相关（P<0.05），与miR-145-5p水平呈负相关（P<0.05）。复发组血清lncRNA MALAT-1水平明显高于非复发组（P<0.05）,miR-145-5p水平低于非复发组（P<0.05）。血清CRP、lncRNA MALAT-1和miR-145-5p水平升高为UC患者预后的危险因素（P<0.05）。血清lncRNA MALAT-1和miR-145-5p单独、联合预测UC复发的曲线下面积（AUC）分别为0.820、0.841、0.924，联合预测效果更佳(Z联合-lncRNA MALAT-1=2.614,P=0.009;Z联合-miR-145-5p=2.563,P=0.010)。结论 UC患者血清lncRNA MALAT-1和miR-145-5p水平变化与疾病活动度、肠道菌群分布显著相关，且二者联合检测对预测复发具有较高临床价值。 |
| 英文摘要: |
| Objective To explore the relationship between serum long non coding RNA metastasis-associated lung adenocarcinoma transcript 1（lncRNA MALAT-1）, miR-145-5p and disease activity, gut microbiota distribution, and recurrence in patients with ulcerative colitis（UC）. Methods A total of 122 patients with ulcerative colitis（UC） who were treated in the Second Gastroenterology Department of Handan Traditional Chinese Medicine Hospital from January 2021 to December 2023 were selected to form the UC group, and were assigned into a non recurrent group（92 cases） and a recurrent group（30 cases） according to their recurrence. Meantime, 100 healthy individuals who underwent physical checkups were recruited as the control group. The Mayo score was used to assess disease activity. The 16 srRNA sequencing technology was used to detect the gut microbiota in two groups, and the relative abundance of gut microbiota at the phylum and genus levels was compared between two groups. Real-time fluorescence quantitative PCR was used to measure serum lncRNA MALAT-1 and miR-145-5p. Pearson method was used to discuss the correlation between gut microbiota and serum lncRNA MALAT-1 and miR-145-5p in UC patients. The ENCORI website was used to predict whether there was a targeted relationship between the two. Multifactorial logistic regression analysis was performed to assess the prognostic influences in UC patients. Receiver operating characteristic（ROC） curves were used to analyze the predictive value of serum lncRNA MALAT-1 and miR-145-5p for recurrence of UC patients. Results The UC group had conspicuously higher serum lncRNA MALAT-1（P<0.05） and clearly lower miR-145-5p than the control group（P<0.05）. There were targeted binding sites for lncRNA MALAT-1 and miR-145-5p, and serum lncRNA MALAT-1 and miR-145-5p of UC patients were negatively correlated（r=-0.536, P<0.05）. Patients in moderate and severe active phase had clearly higher serum lncRNA MALAT-1（P<0.05） and conspicuously lower miR-145-5p than patients in mild active phase and remission phase（P<0.05）. At the phylum level, UC patients had conspicuously lower relative abundance of Firmicutes（P<0.05） and conspicuously higher relative abundance of Proteobacteria（P<0.05）; at the genus level, the relative abundances of Lactobacillus and Bifidobacterium decreased markedly（P<0.05）, while the relative abundances of Enterococcus and Escherichia coli increased evidently（P<0.05）. Lactobacillus and Bifidobacterium were negatively correlated with serum lncRNA MALAT-1（P<0.05） and positively correlated with miR-145-5p（P<0.05）; the Enterococcus and Escherichia coli were positively correlated with serum lncRNA MALAT-1（P<0.05） and negatively correlated with miR-145-5p（P<0.05）. The recurrent group had conspicuously higher serum lncRNA MALAT-1（P<0.05） and conspicuously lower miR-145-5p than the non recurrent group（P<0.05）. Elevated levels of serum CRP, lncRNA MALAT-1, and miR-145-5p were prognostic risk factors for UC patients（P<0.05）. The area under the curve（AUC） of serum lncRNA MALAT-1 and miR-145-5p alone and their joint for predicting UC recurrence was 0.820, 0.841, and 0.924, respectively. The joint prediction effect was better(Zjoint-lncRNA MALAT-1=2.614, P=0.009, Zjoint-miR-145-5p=2.563, P=0.010). Conclusion The changes in serum lncRNA MALAT-1 and miR-145-5p in UC patients are clearly correlated with disease activity and gut microbiota distribution. And the joint detection of the two has high clinical value in predicting recurrence. |
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