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| 罗哌卡因调控cGAS-STING通路对卵巢癌细胞增殖凋亡和侵袭的影响 |
| Impacts of ropivacaine on proliferation, apoptosis, and invasion of ovarian cancer cells by regulating cGAS-STING pathway |
| 投稿时间：2025-02-17 |
| DOI：10.3969/j.issn.1000-0399.2026.02.002 |
| 中文关键词: 罗哌卡因 cGAS-STING通路 卵巢癌 增殖 凋亡 侵袭 |
| 英文关键词: Ropivacaine cGAS-STING pathway Ovarian cancer Proliferation Apoptosis Invasion |
| 基金项目:衡水市科学技术局2024年度第一批科技计划项目(编号:2024014004Z) |
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| 中文摘要: |
| 目的 探讨罗哌卡因（ROP）调控cGAS-STING通路对卵巢癌（OC）细胞增殖、凋亡和侵袭的影响。方法 体外培养人卵巢癌细胞SKOV3，用0、12.5、25、50、100、200 nmol/L的ROP处理，利用四甲基偶氮唑蓝比色法检测不同浓度ROP对SKOV3细胞活性的影响；将SKOV3细胞随机分为对照组、低剂量ROP组（25 nmol/L,ROP-L组）、中剂量ROP组（50 nmol/L,ROP-M组）、高剂量ROP组（100 nmol/L,ROP-H组）和ROP-H+cGAS抑制剂（RU.521）组（100 nmol/L ROP+1μmol/L RU.521）；通过5-乙炔基-2，脱氧尿嘧啶核苷（EdU）染色法检测各组SKOV3细胞增殖情况，流式细胞术检测各组SKOV3细胞凋亡情况，Transwell实验检测各组SKOV3细胞侵袭能力；建立裸鼠移植瘤模型，并测量肿瘤重量和体积；实时荧光定量逆转录聚合酶链式反应（qRT-PCR）和Western blot检测各组SKOV3细胞和肿瘤组织中cGAS、STING mRNA及其蛋白表达量。结果 与对照组相比，ROP-L组、ROP-M组和ROP-H组SKOV3细胞增殖能力、侵袭能力降低，细胞凋亡率、cGAS、STING mRNA和蛋白表达量升高（P<0.05）；与ROP-H组相比，ROP-H+RU.521组SKOV3细胞增殖能力、侵袭能力上升，细胞凋亡率、cGAS、STING mRNA和蛋白表达量下降（P<0.05）；体内移植瘤实验结果显示：与模型组相比，ROP处理组肿瘤重量和体积减小，cGAS、STING mRNA和蛋白表达量升高（P<0.05）。结论 ROP可能通过激活cGAS-STING通路抑制癌细胞增殖、侵袭，促进癌细胞凋亡，从而改善OC。 |
| 英文摘要: |
| Objective To investigate the impacts of ropivacaine(ROP) on proliferation, apoptosis, and invasion of ovarian cancer(OC) cells by regulating cGAS-STING pathway. Methods Human ovarian cancer cells SKOV3 were cultured in vitro and treated with ROP at concentrations of 0, 15, 25, 50, 100, and 200 nmol/L. Then, the effects of different concentrations of ROP on SKOV3 cell activity were detected using methylthiazolyldiphenyl-tetrazolium bromide(MTT) colorimetric method. SKOV3 cells were stochastically grouped into the control group, low-dose ROP group(25 nmol/L, ROP-L group), medium-dose ROP group(50 nmol/L, ROP-M group), high-dose ROP group(100 nmol/L, ROP-H group), and ROP-H+cGAS inhibitor(RU. 521) group(100 nmol/L ROP+1 μmol/L RU. 521). The 5-ethynyl-2, deoxyuridine(EdU) staining method was used to detect the proliferation of SKOV3 cells in each group. Flow cytometry was used to detect apoptosis of SKOV3 cells in each group. Transwell experiment was used to detect the invasion ability of SKOV3 cells in each group. The nude mouse transplant tumor model was established, and tumor weight and volume were measured. Real time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR) and Western blot were used to detect cGAS, STING mRNAs and proteins in SKOV3 cells and tumor tissues of each group. Results For the control group, the ROP-L group, ROP-M group, and ROP-H group showed a prominent decrease in SKOV3 cell proliferation ability and invasion ability, and a prominent increase in cell apoptosis rate, cGAS, STING mRNAs and proteins(P<0.05). For the ROP-H group, the ROP-H+RU.521 group showed a prominent increase in SKOV3 cell proliferation ability and invasion ability, but a prominent decrease in cell apoptosis rate, cGAS, STING mRNAs and proteins(P<0.05). The results of in vivo tumor transplantation experiments showed that for the model group, the ROP treatment groups had prominently reduced tumor weight and volume, and prominently increased cGAS, STING mRNAs and proteins(P<0.05). Conclusion ROP may inhibit cancer cell proliferation and invasion, promote cancer cell apoptosis, and improve OC by activating cGAS-STING pathway. |
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