| Objective To explore the correlation between serum heme oxygenase-1（HO-1）, Runt transcription factor 2（Runx2） levels and abdominal aortic calcification in peritoneal dialysis patients. Methods From July 2022 to July 2024, 181 peritoneal dialysis patients admitted to the Nephrology Department of Handan Central Hospital were enrolled. According to whether abdominal aortic calcification occurred, 94 cases were included into a calcification group and 87 cases were included into a non-calcification group. Another 70 healthy people were selected as the control group. Data such as gender, age, dialysis duration, systolic blood pressure, and diastolic blood pressure were collected. The serum levels of HO-1 and Runx2 were detected by enzyme-linked immunosorbent assay. According to the degree of calcification, the calcification group was assigned into severe calcification group（n=38） and mild calcification group（n=56）. The levels of serum HO-1, Runx2, and aortic calcification score were analyzed and compared between the two groups. Pearson correlation analysis was performed to examine the relationship between serum HO-1 and Runx2 levels and age, duration of dialysis, serum phosphorus, and triglycerides（TG） in patients undergoing peritoneal dialysis with abdominal aortic calcification. Spearman correlation analysis was conducted to assess the association between serum HO-1 and Runx2 levels and aortic calcification scores in these patients. Logistic regression was applied to analyze the factors influencing the occurrence of abdominal aortic calcification in peritoneal dialysis patients. Receiver operating characteristic curve was applied to analyze the value of serum HO-1 and Runx2 in predicting abdominal aortic calcification in peritoneal dialysis patients. Results Patients undergoing peritoneal dialysis exhibited lower levels of urine output, hemoglobin, albumin, serum calcium, total cholesterol, and serum HO-1 compared to the control group（P<0.05）. Conversely, they demonstrated higher levels of systolic blood pressure, fasting blood glucose, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, serum creatinine, serum phosphorus, TG, parathyroid hormone, and serum Runx2 compared to the control group（P<0.05）. The age, dialysis duration, phosphorus, TG, and serum Runx2 level in the calcification group were significantly higher than those in the non-calcification group（P<0.05）, while serum HO-1 level was significantly lower than that in the non-calcification group（P<0.05）. Among them, the serum HO-1 level in the severe calcification group was significantly lower than that in the mild calcification group（P<0.05）, while the serum Runx2 level and aortic calcification score were significantly higher than those in the mild calcification group（P<0.05）. In patients undergoing peritoneal dialysis with abdominal aortic calcification, serum HO-1 level was negatively correlated with age, dialysis duration, phosphorus, TG, and aortic calcification score（P<0.05）, while serum Runx2 level was positively correlated with age, dialysis duration, phosphorus, TG, and aortic calcification score（P<0.05）. Age, phosphorus, and Runx2 were risk factors for abdominal aortic calcification in peritoneal dialysis patients, while HO-1 was a protective factor（P<0.05）. The area under the curve of the combination of serum HO-1 and Runx in predicting abdominal aortic calcification in peritoneal dialysis patients was 0.920, which was superior to single detection(ZHO-1-combination=3.365, P=0.001, ZRunx2-combination=2.789, P=0.005). Conclusion The serum HO-1 level is decreased and Runx2 level is increased in patients with abdominal aortic calcification during peritoneal dialysis. Serum HO-1 and Runx2 are associated with abdominal aortic calcification in peritoneal dialysis patients, and the combination of the two has a high value in predicting the occurrence of abdominal aortic calcification in patients. |
